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Literature summary for 4.2.2.18 extracted from

  • Wang, X.; Yu, S.; Zhang, T.; Jiang, B.; Mu, W.
    Identification of a recombinant inulin fructotransferase (difructose dianhydride III forming) from Arthrobacter sp. 161MFSha2.1 with high specific activity and remarkable thermostability (2015), J. Agric. Food Chem., 63, 3509-3515 .
    View publication on PubMed
Search for more literature for 4.2.2.18

Application

Application Comment Organism
synthesis conversion ratio of inulin to DFA III reaches 81% when 100 g/l inulin is catalyzed by 80 nM recombinant enzyme for 20 min at pH 6.5 and 55°C Arthrobacter sp. 161MFSha2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arthrobacter sp. 161MFSha2.1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
 gel fitlration Arthrobacter sp. 161MFSha2.1

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. 161MFSha2.1 WP_018778058
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2391
-
 pH 6.5, 55°C Arthrobacter sp. 161MFSha2.1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin
-
 Arthrobacter sp. 161MFSha2.1 di-D-fructofuranose 1,2':2,3' dianhydride + ?
-
 ?
nystose smallest substrate recognized Arthrobacter sp. 161MFSha2.1 di-D-fructofuranose 1,2':2,3' dianhydride + sucrose
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 43000, SDS-PAGE Arthrobacter sp. 161MFSha2.1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 stable for up to 4 h Arthrobacter sp. 161MFSha2.1