Literature summary for 4.2.1.92 extracted from

Nilsson, A.K.; Fahlberg, P.; Johansson, O.N.; Hamberg, M.; Andersson, M.X.; Ellerstroem, M.
The activity of hydroperoxide lyase 1 regulates accumulation of galactolipids containing 12-oxo-phytodienoic acid in Arabidopsis (2016), J. Exp. Bot., 67, 5133-5144 .

Cloned(Commentary)

Cloned (Comment)	Organism
genetic mapping and quantitative real-time PCR expression analysis of HPL1 in plants of diverse accessions, e.g. accessions C24 and Ler display large difference in HPL1 expression, overview. Transformation of accession Col-0 plants with the C24 HPL1 allele under transcriptional regulation of the 35S promoter revealing a strong negative correlation between HPL1 expression and arabidopside accumulation after tissue damage. A quantitative trait loci analysis of an F2 population created from a cross between C24 and Col-0 locates a region on chromosome four strongly linked to the capacity to form arabidopsides	Arabidopsis thaliana

Cloned (Comment)

Organism

genetic mapping and quantitative real-time PCR expression analysis of HPL1 in plants of diverse accessions, e.g. accessions C24 and Ler display large difference in HPL1 expression, overview. Transformation of accession Col-0 plants with the C24 HPL1 allele under transcriptional regulation of the 35S promoter revealing a strong negative correlation between HPL1 expression and arabidopside accumulation after tissue damage. A quantitative trait loci analysis of an F2 population created from a cross between C24 and Col-0 locates a region on chromosome four strongly linked to the capacity to form arabidopsides

Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-
thylakoid membrane	-	Arabidopsis thaliana	42651	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid	Arabidopsis thaliana	-	(Z)-3-hexenal + 12-oxo-9-dodecenoic acid	-	?
additional information	Arabidopsis thaliana	galactoplid bound hydroperoxides are substrates for CYP74 members	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	B3LF83	accessions C24 and Col-0	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid	-	Arabidopsis thaliana	(Z)-3-hexenal + 12-oxo-9-dodecenoic acid	-	?
additional information	galactoplid bound hydroperoxides are substrates for CYP74 members	Arabidopsis thaliana	?	-	?

Synonyms

Synonyms	Comment	Organism
HPL1	-	Arabidopsis thaliana
hydroperoxide lyase 1	-	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	wounding induces galactolipids and the enzyme activity	up

General Information

General Information	Comment	Organism
metabolism	enzyme HPL1 competes with allene oxide synthase (AOS) for lipid-bound hydroperoxide fatty acids. The last step in the synthesis of galactolipid-bound OPDA and dnOPDA from unstable allene oxides is exclusively enzyme-catalyzed and not the result of spontaneous cyclization. All steps in arabidopside biosynthesis are enzyme-dependent and apparently all reactions can take place with substrates being esterified to galactolipids	Arabidopsis thaliana
additional information	transformation of Col-0 plants with the C24 HPL1 allele under transcriptional regulation of the 35S promoter reveals a strong negative correlation between HPL1 expression and arabidopside accumulation after tissue damage. A quantitative trait loci analysis of an F2 population created from a cross between C24 and Col-0 locates a region on chromosome four strongly linked to the capacity to form arabidopsides	Arabidopsis thaliana
physiological function	the activity of hydroperoxide lyase 1 (HPL1) regulates the accumulation of galactolipids containing 12-oxo-phytodienoic acid in Arabidopsis thaliana. Galactolipids containing esters of 12-oxo-phytodienoic acid (OPDA) and dinor-12-oxo-phytodienoic acid (dnOPDA) are referred to as arabidopsides (chloroplast membrane lipids containing (dn)OPDA) and accumulate in response to abiotic and biotic stress. Expression analysis of enzyme HPL1 shows large differences in transcript abundance between accessions C24 and Col-0. Accession C24 is identified as a poor accumulator of arabidopsides whereas the commonly used accession Col-0 is found to accumulate comparably large amounts of arabidopsides in response to tissue damage	Arabidopsis thaliana