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Literature summary for 4.2.1.92 extracted from

  • Toporkova, Y.Y.; Gorina, S.S.; Bessolitsyna, E.K.; Smirnova, E.O.; Fatykhova, V.S.; Bruehlmann, F.; Ilyina, T.M.; Mukhtarova, L.S.; Grechkin, A.N.
    Double function hydroperoxide lyases/epoxyalcohol synthases (CYP74C) of higher plants identification and conversion into allene oxide synthases by site-directed mutagenesis (2018), Biochim. Biophys. Acta, 1863, 369-378 .
    View publication on PubMed
Search for more literature for 4.2.1.92

Cloned(Commentary)

Cloned (Comment) Organism
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami(DE3)pLysS B Glycine max
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS Cucumis sativus
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta-gami(DE3)pLysS B Medicago truncatula
CYP74C1_CS gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)pLysS Cucumis sativus
CYP74C4_ST gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)pLysS Solanum tuberosum
sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Cucumis melo

Protein Variants

Protein Variants Comment Organism
L93F/G283A site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme Solanum tuberosum
L93F/G283A site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme. The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis sativus
L97F site-directed mutagenesis, L97F/TCFNSF, multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually do not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid (9-HPOD). The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Medicago truncatula
L97F site-directed mutagenesis, the mutant shows altered substrate specificity and decreased activity compared to the wild-type enzyme Solanum tuberosum
L98F/A287G site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme Solanum tuberosum
L98F/A287G site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme. The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis sativus
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry Cucumis sativus
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry Medicago truncatula
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry. All three mutant forms preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid. Multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually did not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid (9-HPOD) Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis sativus
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Glycine max
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Solanum tuberosum
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Medicago truncatula
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis sativus
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis melo
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Cucumis sativus
-
 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Glycine max
-
 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Medicago truncatula
-
 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Solanum tuberosum
-
 (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Cucumis sativus
-
 (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Cucumis melo
-
 (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Solanum tuberosum
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Cucumis sativus
-
 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Glycine max
-
 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Cucumis melo
-
 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Medicago truncatula
-
 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Cucumis sativus
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Glycine max
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Cucumis melo
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Medicago truncatula
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
additional information Cucumis sativus reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview ?
-
 ?
additional information Cucumis melo reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview ?
-
 ?
additional information Glycine max reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview ?
-
 ?
additional information Solanum tuberosum reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview ?
-
 ?
additional information Cucumis sativus reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview ?
-
 ?
additional information Medicago truncatula reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cucumis melo Q93XR3
-
-
Cucumis sativus A0A0A0K2U8
-
-
Cucumis sativus U5YTM6
-
-
Glycine max M4WFN0
-
-
Medicago truncatula Q7X9B3
-
-
Solanum tuberosum C7ENW3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cucumis melo
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cucumis sativus
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta-gami(DE3)pLysS B by nickel affinity chromatography Medicago truncatula
recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cucumis sativus
recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Solanum tuberosum
recombinant His-tagged wild-type enzyme from Escherichia coli strain Rosetta-gami(DE3)pLysS B by nickel affinity chromatography Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Cucumis sativus (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Glycine max (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Solanum tuberosum (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Medicago truncatula (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Cucumis sativus (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ?
-
 ?
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Cucumis melo (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Cucumis sativus 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Glycine max 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Medicago truncatula 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Solanum tuberosum (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Cucumis sativus (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Cucumis melo (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Solanum tuberosum (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Cucumis sativus 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Glycine max 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Cucumis melo 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Medicago truncatula 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Cucumis sativus (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Glycine max (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Cucumis melo (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Medicago truncatula (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview Cucumis sativus ?
-
 ?
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview Cucumis melo ?
-
 ?
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview Cucumis sativus ?
-
 ?
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview Glycine max ?
-
 ?
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview Solanum tuberosum ?
-
 ?
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview Medicago truncatula ?
-
 ?
additional information no activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Solanum tuberosum ?
-
 ?
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview. No activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Solanum tuberosum ?
-
 ?
additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview. No activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Cucumis melo ?
-
 ?

Subunits

Subunits Comment Organism
More three-dimensional modelling of CYP74s and structure comparisons Cucumis sativus
More three-dimensional modelling of CYP74s and structure comparisons Glycine max
More three-dimensional modelling of CYP74s and structure comparisons Solanum tuberosum
More three-dimensional modelling of CYP74s and structure comparisons Cucumis melo
More three-dimensional modelling of CYP74s and structure comparisons Medicago truncatula

Synonyms

Synonyms Comment Organism
9/13 hydroperoxide lyase
-
 Medicago truncatula
CmHPL
-
 Cucumis melo
Csa_7G075590
-
 Cucumis sativus
CYP74C13_GM
-
 Glycine max
CYP74C13_MT
-
 Medicago truncatula
CYP74C1_CS
-
 Cucumis sativus
CYP74C2
-
 Cucumis melo
CYP74C31
-
 Cucumis sativus
CYP74C4_ST
-
 Solanum tuberosum
fatty acid 9-hydroperoxide lyase UniProt Cucumis sativus
fatty acid 9-hydroperoxide lyase UniProt Cucumis melo
fatty acid hydroperoxide lyase UniProt Solanum tuberosum
fatty acid hydroperoxide lyase UniProt Medicago truncatula
HPL
-
 Cucumis sativus
HPL
-
 Glycine max
HPL
-
 Solanum tuberosum
HPL
-
 Cucumis melo
HPL
-
 Medicago truncatula
HPL1
-
 Cucumis sativus
HPL1
-
 Medicago truncatula
hydroperoxide lyase
-
 Cucumis sativus
hydroperoxide lyase
-
 Glycine max
hydroperoxide lyase
-
 Solanum tuberosum
hydroperoxide lyase
-
 Cucumis melo
hydroperoxide lyase
-
 Medicago truncatula
MtrunA17_Chr4g0034141
-
 Medicago truncatula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Cucumis sativus
23
-
 assay at Glycine max
23
-
 assay at Solanum tuberosum
23
-
 assay at Cucumis melo
23
-
 assay at Medicago truncatula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
173.8
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Glycine max
187.1
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme Medicago truncatula
228.6
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Glycine max
290
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Glycine max
337.6
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
356.1
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme Cucumis sativus
375.4
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
377.2
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
455.3
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
471.4
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme Medicago truncatula
530.9
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
550
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme Medicago truncatula
571.4
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme Medicago truncatula
596.4
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
600
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Solanum tuberosum
619.8
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme Cucumis sativus
676.4
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
712.7
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme Cucumis sativus
732.4
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme Cucumis sativus
752.4
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
768.9
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Medicago truncatula
773.3
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Medicago truncatula
829.1
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme Cucumis sativus
867.7
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Medicago truncatula
913.3
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Medicago truncatula
949.6
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme Cucumis sativus
997
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme Cucumis sativus
1011.6
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Solanum tuberosum
1115.9
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Solanum tuberosum
1587.5
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme Cucumis sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, the reaction mixture acidifies to pH 6.0 Cucumis sativus
7
-
 assay at, the reaction mixture acidifies to pH 6.0 Glycine max
7
-
 assay at, the reaction mixture acidifies to pH 6.0 Solanum tuberosum
7
-
 assay at, the reaction mixture acidifies to pH 6.0 Cucumis melo
7
-
 assay at, the reaction mixture acidifies to pH 6.0 Medicago truncatula

General Information

General Information Comment Organism
evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Cucumis sativus
evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Glycine max
evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Solanum tuberosum
evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Cucumis melo
evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Medicago truncatula
malfunction the mutant enzyme preferentially utilises 9-hydroperoxylinoleic acid Cucumis sativus
malfunction the mutant enzyme preferentially utilises 9-hydroperoxylinoleic acid. Multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually do not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with 9-hydroperoxylinoleic acid (9-HPOD) Medicago truncatula
additional information three-dimensional modelling of CYP74s and structure comparisons Cucumis sativus
additional information three-dimensional modelling of CYP74s and structure comparisons Glycine max
additional information three-dimensional modelling of CYP74s and structure comparisons Solanum tuberosum
additional information three-dimensional modelling of CYP74s and structure comparisons Cucumis melo