BRENDA - Enzyme Database show
show all sequences of 4.2.1.79

2-Methylcitrate dehydratase, a new enzyme of propionate metabolism in Saccharomycopsis lipolytica

Aoki, H.; Nakahara, T.; Tabuchi, T.; Curr. Dev. Yeast Res. , Proc. Int. Yeast Symp. (Stewart, G. G. , Russel, I. , eds. ) Pergamon, Toronto , 435-439 (1980)
No PubMed abstract available

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Yarrowia lipolytica
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
78000
79000
gel filtration
Yarrowia lipolytica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
Yarrowia lipolytica
methylcitric acid cycle, propionyl-CoA oxidation
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Yarrowia lipolytica
-
formerly Saccaromycopsis lipolytica
-
Purification (Commentary)
Commentary
Organism
-
Yarrowia lipolytica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.08
-
-
Yarrowia lipolytica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
i.e. 2-methylcitrate
5896
Yarrowia lipolytica
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
-
2-Hydroxybutane-1,2,3-tricarboxylate
methylcitric acid cycle, propionyl-CoA oxidation
5896
Yarrowia lipolytica
?
-
-
-
-
additional information
not: citrate, threo-DL-isocitrate, threo-D-2-methylisocitrate, DL-homocitrate, DL-citramalate
5896
Yarrowia lipolytica
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Yarrowia lipolytica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.5
-
Yarrowia lipolytica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Yarrowia lipolytica
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
78000
79000
gel filtration
Yarrowia lipolytica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
Yarrowia lipolytica
methylcitric acid cycle, propionyl-CoA oxidation
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Yarrowia lipolytica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.08
-
-
Yarrowia lipolytica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Hydroxybutane-1,2,3-tricarboxylate
i.e. 2-methylcitrate
5896
Yarrowia lipolytica
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
-
-
-
2-Hydroxybutane-1,2,3-tricarboxylate
methylcitric acid cycle, propionyl-CoA oxidation
5896
Yarrowia lipolytica
?
-
-
-
-
additional information
not: citrate, threo-DL-isocitrate, threo-D-2-methylisocitrate, DL-homocitrate, DL-citramalate
5896
Yarrowia lipolytica
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Yarrowia lipolytica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.5
-
Yarrowia lipolytica
Other publictions for EC 4.2.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747131
Reddick
First biochemical characteriz ...
Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Escherichia coli K12
Biochemistry
56
5698-5711
2017
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1
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4
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8
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8
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2
2
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749113
Rocco
The PrpF protein of Shewanell ...
Bacillus subtilis 168, Bacillus subtilis, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720
PLoS ONE
12
e0188130
2017
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3
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1
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5
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9
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1
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11
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3
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3
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3
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1
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5
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1
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11
-
3
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3
-
-
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6
6
-
-
-
728336
Limenitakis
The 2-methylcitrate cycle is i ...
Toxoplasma gondii, Toxoplasma gondii RH
Mol. Microbiol.
87
894-908
2013
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1
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2
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2
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2
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704332
Rocco
In Salmonella enterica, 2-meth ...
Salmonella enterica
J. Bacteriol.
192
771-778
2010
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4
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4
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713880
Peplinski
Investigations on the microbia ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Appl. Microbiol. Biotechnol.
88
1145-1159
2010
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1
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24
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1
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1
1
2
2
1
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706675
Santos
Adaptation to beta-myrcene cat ...
Pseudomonas sp., Pseudomonas sp. M1
Proteomics
9
5101-5111
2009
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2
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2
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681801
Upton
Role of the methylcitrate cycl ...
Mycolicibacterium smegmatis
Microbiology
153
3973-3982
2007
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2
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4
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2
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672679
Mohamed Fahmy Gad El-Rab
Effects of cadmium stress on g ...
Rhodobacter capsulatus, Rhodobacter capsulatus B10
Biosci. Biotechnol. Biochem.
70
2394-2402
2006
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6
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1
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681995
Gould
Dual role of isocitrate lyase ...
Mycobacterium tuberculosis
Mol. Microbiol.
61
940-947
2006
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3
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651735
Grimek
The acnD genes of Shewenella o ...
Shewanella oneidensis, Vibrio cholerae
J. Bacteriol.
186
454-462
2004
4
-
2
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2
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2
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6
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1
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1
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8
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1
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4
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2
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1
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1
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8
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1
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488175
Brock
Oxidation of propionate to pyr ...
Escherichia coli
Eur. J. Biochem.
269
6184-6194
2002
1
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1
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1
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1
1
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3
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1
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1
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4
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1
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1
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1
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4
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1
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1
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649188
Gulick
Pentaerythritol propoxylate: a ...
Salmonella enterica
Acta Crystallogr. Sect. D
58
306-309
2002
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1
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1
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653158
Blank
AcnC of Escherichia coli is a ...
Escherichia coli
Microbiology
148
133-146
2002
1
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1
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1
1
1
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1
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1
1
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11
1
1
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1
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650059
Horswill
In vitro conversion of propion ...
Salmonella enterica
Biochemistry
40
4703-4713
2001
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1
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5897
Aoki
-
Purification and properties of ...
Yarrowia lipolytica
Agric. Biol. Chem.
45
2831-2837
1981
1
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5898
Tabuchi
-
2-Methylcitrate dehydratase, a ...
Yarrowia lipolytica
Agric. Biol. Chem.
45
2823-2929
1981
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1
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5896
Aoki
-
2-Methylcitrate dehydratase, a ...
Yarrowia lipolytica
Curr. Dev. Yeast Res. , Proc. Int. Yeast Symp. (Stewart, G. G. , Russel, I. , eds. ) Pergamon, Toronto
435-439
1980
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