Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no bound cofactor | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Butane-2,3-dione | 20% residual activity after 45 min at 40 mM | Euglena gracilis | |
diethyl dicarbonate | 10% residual activity after 5 min at 0.41 mM | Euglena gracilis | |
NaN3 | 25-30% residual activity with 1.0 mM | Euglena gracilis | |
Phenylglyoxal | 50% residual activity after 1 min at 0.3 mM | Euglena gracilis | |
pyridoxal 5'-phosphate | - |
Euglena gracilis | |
uroporphyrinogen III | product inhibition | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | 0.04 | Hydroxymethylbilane | - |
Euglena gracilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ions present in enzyme | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
SDS-PAGE | Euglena gracilis |
38500 | - |
SDS-PAGE, gel filtration | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hydroxymethylbilane | Euglena gracilis | synthesis of uroporphyrinogen III, key intermediate for biosynthesis of tetrapyrrolic compounds like chlorophylls, hemes, cytochromes and vitamin B12 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Euglena gracilis |
Storage Stability | Organism |
---|---|
-70¦C, several weeks | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hydroxymethylbilane | - |
Euglena gracilis | Uroporphyrinogen III | - |
? | |
Hydroxymethylbilane | synthesis of uroporphyrinogen III, key intermediate for biosynthesis of tetrapyrrolic compounds like chlorophylls, hemes, cytochromes and vitamin B12 | Euglena gracilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE, gel filtration | Euglena gracilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay at | Euglena gracilis |