Any feedback?
Literature summary for 4.2.1.67 extracted from
- D-Fuconate dehydratase (1975), Methods Enzymol., 42C, 305-308.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 2.8 mM, activation | Pseudomonas sp. |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | 13.3 mM, 18% residual activity | Pseudomonas sp. | |
| CuSO4 | 13.3 mM, 29% residual activity | Pseudomonas sp. | |
| EDTA | - |
Pseudomonas sp. | |
| FeSO4 | 13.3 mM, 37% residual activity | Pseudomonas sp. | |
| NiCl2 | 13.3 mM, 8% residual activity | Pseudomonas sp. | |
| ZnCl2 | 13.3 mM, complete inactivation | Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
D-fuconate | - |
Pseudomonas sp. | |
| 4.3 | - |
L-arabonate | - |
Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimum: 20 mM, required | Pseudomonas sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.33 | - |
- |
Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 10% loss of activity after 1 week | Pseudomonas sp. |
| frozen, 24 h, 50% loss of activity | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Fuconate | - |
Pseudomonas sp. | 2-Dehydro-3-deoxy-D-fuconate + H2O | - |
? | |
| L-Arabonate | - |
Pseudomonas sp. | 2-Keto-3-deoxy-L-arabonate + H2O | - |
? | |
| additional information | not: L-fuconate, D-arabonate, D-galactonate, 6-iodo-6-deoxy-D-galactonate, D-ribonate, D-xylonate, D-lyxonate, D-mannonate, L-rhamnonate, D-gluconate, L-galactonate, L-gluconate, D-galactarate, D-glucarate, D-glucuronate, D-galacturonate, cellobionate | Pseudomonas sp. | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 7.4 | - |
Pseudomonas sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
