Protein Variants | Comment | Organism |
---|---|---|
D135135N/E136Q | the turnover number for dTDP-6-fluoro-6-deoxyglucose is 340fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 204fold lower than that of the wild-type enzyme | Escherichia coli |
D135A | the steady-state rate of conversion of dTDP-6-fluoro-6-deoxyglucose to dTDP-4-keto-6-deoxyglucose is identical to that of wild-type. The turnover number for dTDP-6-fluoro-6-deoxyglucose is 1.5fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 223fold lower than that of the wild-type enzyme | Escherichia coli |
D135N | the steady-state rate of conversion of dTDP-6-fluoro-6-deoxyglucose to dTDP-4-keto-6-deoxyglucose is identical to that of wild-type. The turnover number for dTDP-6-fluoro-6-deoxyglucose is 1.2fold higher than that of the wild-type enzyme, the turnover number for dTDP-glucose is 124fold lower than that of the wild-type enzyme | Escherichia coli |
E136A | the turnover number for dTDP-6-fluoro-6-deoxyglucose is 690fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 288fold lower than that of the wild-type enzyme | Escherichia coli |
E136Q | the turnover number for dTDP-6-fluoro-6-deoxyglucose is 69fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 67fold lower than that of the wild-type enzyme | Escherichia coli |
E198Q | the turnover number for dTDP-6-fluoro-6-deoxyglucose is 190fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 258fold lower than that of the wild-type enzyme | Escherichia coli |
K199M | the turnover number for dTDP-6-fluoro-6-deoxyglucose is 260fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 115fold lower than that of the wild-type enzyme | Escherichia coli |
K199R | the turnover number for dTDP-6-fluoro-6-deoxyglucose is 680fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 288fold lower than that of the wild-type enzyme | Escherichia coli |
Y301F | the turnover number for dTDP-6-fluoro-6-deoxyglucose is 31fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 117fold lower than that of the wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P27830 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-6-fluoro-6-deoxyglucose | substrate undergoes fluoride ion elimination instead of dehydration | Escherichia coli | dTDP-4-keto-6-deoxyglucose + F- | - |
? | |
dTDP-glucose | the conversion takes place in three steps: dehydrogenation to dTDP-4-ketoglucose, dehydration to dTDP-4-ketoglucose-5,6-ene and rereduction of C6 to the methyl group. Asp135 and Glu136 are the acid and base catalysts, respectively of the dehydration step | Escherichia coli | dTDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000064 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme E136A | Escherichia coli | |
0.000065 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme K199R | Escherichia coli | |
0.00013 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme D135N/E136Q | Escherichia coli | |
0.00017 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme K199M | Escherichia coli | |
0.00023 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme E198Q | Escherichia coli | |
0.00064 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme E136Q | Escherichia coli | |
0.0014 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme Y301F | Escherichia coli | |
0.017 | - |
dTDP-glucose | mutant enzyme E136A | Escherichia coli | |
0.017 | - |
dTDP-glucose | mutant enzyme K199R | Escherichia coli | |
0.019 | - |
dTDP-glucose | mutant enzyme E198Q | Escherichia coli | |
0.022 | - |
dTDP-glucose | mutant enzyme D135A | Escherichia coli | |
0.024 | - |
dTDP-glucose | mutant enzyme D135N/E136Q | Escherichia coli | |
0.029 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme D135A | Escherichia coli | |
0.0395 | - |
dTDP-glucose | mutant enzyme D135N | Escherichia coli | |
0.042 | - |
dTDP-glucose | mutant enzyme Y301F | Escherichia coli | |
0.0425 | - |
dTDP-glucose | mutant enzyme K199M | Escherichia coli | |
0.044 | - |
dTDP-6-fluoro-6-deoxyglucose | wild-type enzyme | Escherichia coli | |
0.053 | - |
dTDP-6-fluoro-6-deoxyglucose | mutant enzyme D135N | Escherichia coli | |
0.073 | - |
dTDP-glucose | mutant enzyme E136Q | Escherichia coli | |
4.9 | - |
dTDP-glucose | wild-type enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | tightly bound | Escherichia coli |