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Literature summary for 4.2.1.46 extracted from

  • Matern, H.; Brillinger, G.U.; Pape, H.
    Stoffwechselprodukte von Mikroorganismen. 114. Mitteilung. Thymidin-diphospho-D-glucose oxidoreduktase aus Streptomyces rimosus (1973), Arch. Mikrobiol., 88, 37-48.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate
-
Streptomyces rimosus
TDP competitive inhibitor Streptomyces rimosus
TMP competitive inhibitor Streptomyces rimosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.093
-
dTDPglucose
-
Streptomyces rimosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-glucose Streptomyces rimosus activity increases during stationary growth phase ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces rimosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces rimosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
dTDPglucose Streptomyces rimosus

Storage Stability

Storage Stability Organism
60% loss of activity during storage in 0.025 M potassium phosphate or 0.025 M Tris-HCl at 0¦C for 24 hours, stable for several days at 0¦C and buffer concentrations of 0.25 M or 20% glycerol or 34% sucrose Streptomyces rimosus
stable for several weeks at -20¦C Streptomyces rimosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-glucose
-
Streptomyces rimosus dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?
dTDP-glucose activity increases during stationary growth phase Streptomyces rimosus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
dTDPglucose Streptomyces rimosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9 rapid inactivation at acidic pH Streptomyces rimosus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Streptomyces rimosus