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Literature summary for 4.2.1.28 extracted from
- The N-terminal region of the medium subunit (PduD) packages adenosylcobalamin-dependent diol dehydratase (PduCDE) into the Pdu microcompartment (2011), J. Bacteriol., 193, 5623-5628.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of fusion protein PduD1-18-eGFP from a plasmid in a pduCDE deletion mutant | Salmonella enterica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a fusion protein PduD1-18-eGFP | Salmonella enterica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| propane-1,2-diol | Salmonella enterica | - |
propanal + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by repeated immunoprecipitation on protein A-Sepharose beads, proteins fused to the N-terminus of PduD are encapsulated within the shell of the Pdu MCP | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| propane-1,2-diol | - |
Salmonella enterica | propanal + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| coenzyme B12-dependent diol dehydratase | - |
Salmonella enterica |
| diol dehydratase | - |
Salmonella enterica |
| PduCDE | - |
Salmonella enterica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Salmonella enterica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Salmonella enterica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| adenosylcobalamin | i.e. coenzyme B12, dependent on | Salmonella enterica | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | Salmonella enterica produces a proteinaceous microcompartment for B12-dependent 1,2-propanediol utilization, Pdu MCP. The Pdu MCP consists of catabolic enzymes encased within a protein shell and its function is to sequester propionaldehyde, a toxic intermediate of 1,2-propanediol degradation, overview. A short N-terminal region of the medium subunit, PduD, is required for packaging the coenzyme B12-dependent diol dehydratase, PduCDE, into the lumen of the Pdu MCP. PduD subunit also mediates packaging of itself and other subunits of diol dehydratase, PduC and PduE, into the Pdu MCP | Salmonella enterica |
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Release 2023.1 (January 2023)
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