Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme requires exogenous thiols and zinc ions for optimal activity | Pyrobaculum calidifontis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
expression in Escherichia coli | Homo sapiens |
expression in Escherichia coli | Pyrobaculum calidifontis |
Crystallization (Comment) | Organism |
---|---|
high-resolution structure of the enzyme cocrystallized with a noncovalently bound moiety of the product, porphobilinogen. The pyrrole side-chain amino group is datively bound to the active-site zinc ion and the porphobilinogen carboxylates interact with the enzyme via hydrogen bonds and salt bridges with invariant residues. Comparison of substrate and product complexes from humans, Escherichia coli and the hyperthermophile Pyrobaculum calidifontis | Escherichia coli |
to 3.5 A resolution. Comparison of substrate and product complexes from humans, Escherichia coli and the hyperthermophile Pyrobaculum calidifontis | Pyrobaculum calidifontis |
two monomers per asymmetric unit. In native human ALAD, the A monomer has a ligand resembling the substrate 5-aminolaevulinic acid which is covalently bound by a Schiff base to active-site Lys252 and is held in place by an ordered active-site loop. These features of the active-site structure are disordered or absent in the B subunit. Comparison of substrate and product complexes from humans, Escherichia coli and the hyperthermophile Pyrobaculum calidifontis | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | requires exogenous thiols and zinc ions for optimal activity. 0.7 zinc ions per mol of subunit | Pyrobaculum calidifontis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ACB2 | - |
- |
Homo sapiens | P13716 | - |
- |
Pyrobaculum calidifontis | A3MWV9 | - |
- |
Pyrobaculum calidifontis JCM 11548 | A3MWV9 | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 37676, mass spectroscopy | Pyrobaculum calidifontis |
Synonyms | Comment | Organism |
---|---|---|
ALAD | - |
Homo sapiens |
HemB | - |
Escherichia coli |
Pcal_1709 | locus name | Pyrobaculum calidifontis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life 9 h | Pyrobaculum calidifontis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Pyrobaculum calidifontis |