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Literature summary for 4.2.1.24 extracted from
- Probing the active site of rat porphobilinogen synthase using newly developed inhibitors (2009), Bioorg. Chem., 37, 33-40.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hexa-His-tag, removed by cleavage with thrombin | Rattus sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (4E)-6-oxodec-4-enedioic acid | - |
Rattus sp. |  |
| 1-amino-4-hydroxy-2-butanone | - |
Rattus sp. | |
| 1-amino-4-methoxy-2-butanone | - |
Rattus sp. | |
| 1-amino-5-hydroxy-2-pentanone | - |
Rattus sp. | |
| 4-oxo-pentanenitrile | - |
Rattus sp. | |
| 4-oxo-sebacic acid | - |
Rattus sp. | |
| 5-amino-4-oxopentanenitrile | - |
Rattus sp. | |
| 5-bromo-levulinic acid | - |
Rattus sp. | |
| 5-oxo-hexanoic acid | - |
Rattus sp. | |
| 6-amino-5-oxohexanoic acid | - |
Rattus sp. | |
| EDTA | - |
Rattus sp. | |
| levulinic acid | - |
Rattus sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
5-aminolevulinic acid | expressed from expression vector pET28a+ | Rattus sp. | |
| 2.1 | - |
5-aminolevulinic acid | expressed from expression vector pET28a+, His-tag removed | Rattus sp. | |
| 3.22 | - |
5-aminolevulinic acid | expressed from expression vector pLM1 | Rattus sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Rattus sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| apparent homogeneity (analyzed by SDS-PAGE) | Rattus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.09 | - |
expressed from expression vector pLM1 | Rattus sp. |
| 0.28 | - |
expressed from expression vector pET28a+ | Rattus sp. |
| 0.29 | - |
expressed from expression vector pET28a+, His-tag removed | Rattus sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80øC, one year | Rattus sp. |
| 4øC, one week, without significant change of activity | Rattus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-aminolevulinic acid + 5-aminolevulinic acid | - |
Rattus sp. | porphobilinogen + 2 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5-aminolaevulinic acid dehydratase | - |
Rattus sp. |
| Porphobilinogen synthase | - |
Rattus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Rattus sp. |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.96 | - |
levulinic acid | - |
Rattus sp. | |
| 1.07 | - |
6-amino-5-oxohexanoic acid | - |
Rattus sp. | |
| 2.93 | - |
4-oxo-sebacic acid | - |
Rattus sp. | |
| 3.05 | - |
5-oxo-hexanoic acid | - |
Rattus sp. | |
| 18.48 | - |
5-amino-4-oxopentanenitrile | - |
Rattus sp. | |
| 26.52 | - |
1-amino-4-hydroxy-2-butanone | - |
Rattus sp. | |
| 73.04 | - |
4-oxo-pentanenitrile | - |
Rattus sp. | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 30 | - |
- |
Rattus sp. | 1-amino-4-methoxy-2-butanone | |
| 32.5 | - |
- |
Rattus sp. | 1-amino-5-hydroxy-2-pentanone | |
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