Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
E138A | inactive mutant enzyme | Pseudomonas putida |
E138D | the ratio of turnover number to KM-value is 9fold lower than the wild-type ratio | Pseudomonas putida |
E138Q | the ratio of turnover number to KM-value is 3600fold lower than the wild-type ratio | Pseudomonas putida |
G118E | no activity with crotonyl-CoA, methylcrotonyl-CoA and methylglutaconyl-CoA | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-methylcrotonyl-CoA | - |
Pseudomonas putida | |
crotonyl-CoA | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0291 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, wild-type enzyme | Pseudomonas putida | |
0.043 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, mutant enzyme E138Q | Pseudomonas putida | |
0.08 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, mutant enzyme E138D | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
PA2011 | - |
Pseudomonas putida PA2011 | - |
PA2011 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with crotonyl-CoA and methylcrotonyl-CoA | Pseudomonas putida | ? | - |
? | |
additional information | no activity with crotonyl-CoA and methylcrotonyl-CoA | Pseudomonas putida PA2011 | ? | - |
? | |
trans-3-methylglutaconyl-CoA + H2O | - |
Pseudomonas putida | (S)-3-hydroxymethylglutaryl-CoA | - |
? | |
trans-3-methylglutaconyl-CoA + H2O | - |
Pseudomonas putida PA2011 | (S)-3-hydroxymethylglutaryl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(3S)-methylglutaconyl-CoA hydratase | - |
Pseudomonas putida |
MGCH | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00086 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, mutant enzyme E138Q | Pseudomonas putida | |
0.064 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, mutant enzyme E138D | Pseudomonas putida | |
2.1 | - |
trans-3-methylglutaconyl-CoA | pH 7.9, 25°C, wild-type enzyme | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0317 | - |
3-methylcrotonyl-CoA | pH 7.09, 25°C, wild-type enzyme | Pseudomonas putida | |
0.0949 | - |
crotonyl-CoA | pH 7.9, 25°C, wild-type enzyme | Pseudomonas putida |