Literature summary for 4.2.1.177 extracted from

Liu, J.; Wei, Y.; Lin, L.; Teng, L.; Yin, J.; Lu, Q.; Chen, J.; Zheng, Y.; Li, Y.; Xu, R.; Zhai, W.; Liu, Y.; Liu, Y.; Cao, P.; Ang, E.; Zhao, H.; Yuchi, Z.; Zhang, Y.
Two radical-dependent mechanisms for anaerobic degradation of the globally abundant organosulfur compound dihydroxypropanesulfonate (2020), Proc. Natl. Acad. Sci. USA, 117, 15599-15608 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the enzyme can be activated by anaerobically reconstituted alcohol dehydrogenase HpfH	Klebsiella oxytoca

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Klebsiella oxytoca

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5	-	(2S)-2,3-dihydroxypropane-1-sulfonate	at pH 7.5 and 25°C	Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2S)-2,3-dihydroxypropane-1-sulfonate	Klebsiella oxytoca	-	3-sulfopropionaldehyde + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Klebsiella oxytoca	A0A318FL05	-	-

Purification (Commentary)

Purification (Comment)	Organism
Talon Co2+ column chromatography	Klebsiella oxytoca

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S)-2,3-dihydroxypropane-1-sulfonate	-	Klebsiella oxytoca	3-sulfopropionaldehyde + H2O	-	?

Synonyms

Synonyms	Comment	Organism
(S)-DHPS-dehydratase	-	Klebsiella oxytoca
hpfG	-	Klebsiella oxytoca

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
130	-	(2S)-2,3-dihydroxypropane-1-sulfonate	at pH 7.5 and 25°C	Klebsiella oxytoca

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
26	-	(2S)-2,3-dihydroxypropane-1-sulfonate	at pH 7.5 and 25°C	Klebsiella oxytoca

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Release 2023.1 (January 2023)