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Literature summary for 4.2.1.175 extracted from

  • Dickert, S.; Pierik, A.J.; Linder, D.; Buckel, W.
    The involvement of coenzyme A esters in the dehydration of (R)-phenyllactate to (E)-cinnamate by Clostridium sporogenes (2000), Eur. J. Biochem., 267, 3874-3884.
    View publication on PubMed
Search for more literature for 4.2.1.175

General Stability

General Stability Organism
oxygen sensitive with a half-life of approximately 12 h Clostridium sporogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 46000 (FldA), 1 * 43000 (FldB), 1 * 40000 (FldC), the 46000 Da FldA subunit mediates CoA-transfer to phenyllactate, subunits FldBC mediate dehydration of phenyllactyl-CoA to cinnamoyl-CoA, SDS-PAGE Clostridium sporogenes
43000
-
 1 * 46000 (FldA), 1 * 43000 (FldB), 1 * 40000 (FldC), the 46000 Da FldA subunit mediates CoA-transfer to phenyllactate, subunits FldBC mediate dehydration of phenyllactyl-CoA to cinnamoyl-CoA, SDS-PAGE Clostridium sporogenes
46000
-
 1 * 46000 (FldA), 1 * 43000 (FldB), 1 * 40000 (FldC), the 46000 Da FldA subunit mediates CoA-transfer to phenyllactate, subunits FldBC mediate dehydration of phenyllactyl-CoA to cinnamoyl-CoA, SDS-PAGE Clostridium sporogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-phenyllactoyl-CoA Clostridium sporogenes the enzyme is involved in fermentation of phenylalanine (2E)-3-phenylprop-2-enoyl-CoA + H2O i.e. (E)-cinnamoyl-CoA, (E)-3-phenylacryloyl-CoA ?
(R)-3-phenyllactoyl-CoA Clostridium sporogenes ATCC 3584 the enzyme is involved in fermentation of phenylalanine (2E)-3-phenylprop-2-enoyl-CoA + H2O i.e. (E)-cinnamoyl-CoA, (E)-3-phenylacryloyl-CoA ?

Organism

Organism UniProt Comment Textmining
Clostridium sporogenes Q93AL9 and Q93AL8 Q93AL9: subunit FldB, Q93AL8: subunit FldB
-
Clostridium sporogenes ATCC 3584 Q93AL9 and Q93AL8 Q93AL9: subunit FldB, Q93AL8: subunit FldB
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium sporogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-phenyllactoyl-CoA
-
 Clostridium sporogenes (2E)-3-phenylprop-2-enoyl-CoA + H2O i.e. (E)-cinnamoyl-CoA, (E)-3-phenylacryloyl-CoA ?
(R)-3-phenyllactoyl-CoA the enzyme is involved in fermentation of phenylalanine Clostridium sporogenes (2E)-3-phenylprop-2-enoyl-CoA + H2O i.e. (E)-cinnamoyl-CoA, (E)-3-phenylacryloyl-CoA ?
(R)-3-phenyllactoyl-CoA
-
 Clostridium sporogenes ATCC 3584 (2E)-3-phenylprop-2-enoyl-CoA + H2O i.e. (E)-cinnamoyl-CoA, (E)-3-phenylacryloyl-CoA ?
(R)-3-phenyllactoyl-CoA the enzyme is involved in fermentation of phenylalanine Clostridium sporogenes ATCC 3584 (2E)-3-phenylprop-2-enoyl-CoA + H2O i.e. (E)-cinnamoyl-CoA, (E)-3-phenylacryloyl-CoA ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 46000 (FldA), 1 * 43000 (FldB), 1 * 40000 (FldC), the 46000 Da FldA subunit mediates CoA-transfer to phenyllactate, subunits FldBC mediate dehydration of phenyllactyl-CoA to cinnamoyl-CoA, SDS-PAGE Clostridium sporogenes

Synonyms

Synonyms Comment Organism
(R)-phenyllactyl-CoA dehydratase
-
 Clostridium sporogenes
FldBC
-
 Clostridium sporogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Clostridium sporogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Clostridium sporogenes

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains FAD Clostridium sporogenes
[4Fe-4S]-center contains a [4Fe-4S]2+ cluster Clostridium sporogenes

General Information

General Information Comment Organism
metabolism the enzyme is involved in fermentation of phenylalanine Clostridium sporogenes