Literature summary for 4.2.1.163 extracted from

Adachi, T.; Izumi, A.; Rea, D.; Park, S.Y.; Tame, J.R.; Roper, D.I.
Expression, purification and crystallization of 2-oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C (2006), Acta Crystallogr. Sect. F, 62, 1010-1012.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 6.4% (w/v) polyethylene glycol 20000, 5% (w/v) polyethylene glycol 550 monomethyl ether, 0.7 M potassium thiocyanate, in 50 mM MES pH 5.5	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(4Z)-2-oxohept-4-enedioate + H2O	Escherichia coli	-	(4S)-4-hydroxy-2-oxoheptanedioate	-	r
(4Z)-2-oxohept-4-enedioate + H2O	Escherichia coli C	-	(4S)-4-hydroxy-2-oxoheptanedioate	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P42270	-	-
Escherichia coli C	P42270	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, HisTrap column chromatography, and Superdex 75 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(4Z)-2-oxohept-4-enedioate + H2O	-	Escherichia coli	(4S)-4-hydroxy-2-oxoheptanedioate	-	r
(4Z)-2-oxohept-4-enedioate + H2O	-	Escherichia coli C	(4S)-4-hydroxy-2-oxoheptanedioate	-	r

Synonyms

Synonyms	Comment	Organism
HpcG	-	Escherichia coli
OHED hydratase	-	Escherichia coli

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Release 2023.1 (January 2023)