Literature summary for 4.2.1.156 extracted from

Yew, W.S.; Fedorov, A.A.; Fedorov, E.V.; Almo, S.C.; Gerlt, J.A.
Evolution of enzymatic activities in the enolase superfamily: L-talarate/galactarate dehydratase from Salmonella typhimurium LT2 (2007), Biochemistry, 46, 9564-9577.

Search for more literature for 4.2.1.156

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of the wild type enzyme complexed with L-lyxarohydroxamate, and of the K197A mutant complexed with L-glucarate. Residue Lys 197 functions as the galactarate-specific base and His 328 functions as the L-talarate-specific base	Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants	Comment	Organism
H328A	inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate	Salmonella enterica subsp. enterica serovar Typhimurium
H328N	inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate	Salmonella enterica subsp. enterica serovar Typhimurium
K197A	inactive. Mutation totally eliminates both the dehydration and epimerization activities using both L-talarate and galactarate	Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	L-talarate	pH 8.0, 25°C	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required. Ligands are residues Asp226, Glu252, and Glu278	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	Q8ZL58	bifunctional L-talarate/galactarate dehydratase, activites of ECs 4.2.1.42 and 4.2.1.156	-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720	Q8ZL58	bifunctional L-talarate/galactarate dehydratase, activites of ECs 4.2.1.42 and 4.2.1.156	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-talarate	-	Salmonella enterica subsp. enterica serovar Typhimurium	5-dehydro-4-deoxy-D-glucarate + H2O	the dehydration of L-talarate is accompanied by competing epimerization to galactarate	?
L-talarate	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720	5-dehydro-4-deoxy-D-glucarate + H2O	the dehydration of L-talarate is accompanied by competing epimerization to galactarate	?

Synonyms

Synonyms	Comment	Organism
STM3697	-	Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1	-	L-talarate	pH 8.0, 25°C	Salmonella enterica subsp. enterica serovar Typhimurium

General Information

General Information	Comment	Organism
physiological function	Salmonella typhimurium LT2 can utilize L-talarate as carbon source. Insertional disruption of the gene talarate dehydratase abolishes this phenotype. The disruption also diminishes, but does not eliminate, the ability of the organism to utilize galactarate as carbon source	Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
9.1	-	L-talarate	pH 8.0, 25°C	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)