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Literature summary for 4.2.1.135 extracted from
- The biosynthesis of UDP-D-QuiNAc in Bacillus cereus ATCC 14579 (2015), PLoS ONE, 10, e133790 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Bc3750, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | 77% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus |  |
| UDP-galactose | 51% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus | |
| UDP-GalNAc | 91% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus | |
| UDP-glucose | 81% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus | |
| UTP | 98% inhibition of the recombinant enzyme at 0.2 mM | Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26700 | - |
recombinant His6-tagged enzyme, gel filtration | Bacillus cereus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine | Bacillus cereus | - |
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine | Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | - |
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | Q81A42 | - |
- |
| Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | Q81A42 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | product identification by mass spectrometry and one- and two-dimensional NMR analysis, substrate specificity analysis, overview. None of the UDP-sugars tested including UDP-galactose, UDPGalNAc, UDP-GlcNAc, and UDP-glucose are substrates for recombinant Pdeg | Bacillus cereus | ? | - |
? | |
| additional information | product identification by mass spectrometry and one- and two-dimensional NMR analysis, substrate specificity analysis, overview. None of the UDP-sugars tested including UDP-galactose, UDPGalNAc, UDP-GlcNAc, and UDP-glucose are substrates for recombinant Pdeg | Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | ? | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine | - |
Bacillus cereus | UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine | - |
Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000, recombinant His6-tagged enzyme, SDS-PAGE | Bacillus cereus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bc3750 | - |
Bacillus cereus |
| BC_3750 | - |
Bacillus cereus |
| Pdeg | - |
Bacillus cereus |
| UDP-Glc-NAc 4,6-dehydratase | - |
Bacillus cereus |
| UDP-N-acetylglucosamine C4,6-dehydratase | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 25 | recombinant His6-tagged enzyme | Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
purified recombinant His6-tagged enzyme, 2 min, inactivation | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | recombinant His6-tagged enzyme | Bacillus cereus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Bacillus cereus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzme is involved in the biosynthesis of Bacillus glycans and N-acetylquinovosamine (2-acetamido-2,6-di-deoxy-D-glucose, QuiNAc) | Bacillus cereus |
| physiological function | gene Pdeg encodes an UDP-N-acetylglucosamine C4,6-dehydratase that converts UDP-GlcNAc to UDP-4-keto-4,6-D-deoxy-GlcNAc (UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose). The product is then catalyzed by Preq-encoded UDP-4-reductase that converts UDP-4-keto-4,6-d-deoxy-GlcNAc to UDP-N-acetylquinovosamine | Bacillus cereus |
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