Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus thuringiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, complete loss of activity. More than 95% of activity can be restored in presence of Mg2+ | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
(-)-3-dehydroshikimate | 37°C | Bacillus thuringiensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Bacillus thuringiensis | |
Mg2+ | presence of Mg2+ restores activity after incubation with EDTA | Bacillus thuringiensis | |
Mn2+ | - |
Bacillus thuringiensis | |
additional information | a divalent metal ion is absolutely required | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Bacillus thuringiensis 97-27 | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
complete loss of activity in presence of EDTA. More than 95% of activity can be restored in presence of Mg2+ | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-3-dehydroshikimate | - |
Bacillus thuringiensis | 3,4-dihydroxybenzoate + H2O | - |
? | |
(-)-3-dehydroshikimate | - |
Bacillus thuringiensis 97-27 | 3,4-dihydroxybenzoate + H2O | - |
? | |
additional information | enzyme displays to ionizable groups with pKa1 7.9, and pKa2 9.3 | Bacillus thuringiensis | ? | - |
? | |
additional information | enzyme displays to ionizable groups with pKa1 7.9, and pKa2 9.3 | Bacillus thuringiensis 97-27 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(-)-3-dehydroshikimate dehydratase | - |
Bacillus thuringiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
half-life 10 min | Bacillus thuringiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
(-)-3-dehydroshikimate | 37°C | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.8 | - |
Bacillus thuringiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
less than 15% residual activity | Bacillus thuringiensis |