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Literature summary for 4.2.1.115 extracted from
- FlaA1, a new bifunctional UDP-GlcNAc C6 Dehydratase/C4 reductase from Helicobacter pylori (2000), J. Biol. Chem., 275, 34873-34880.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene flaA1, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), complementation of a Pseudomonas aeruginosa WbpM knockout by expression of His-tagged FlaA1 | Helicobacter pylori |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Helicobacter pylori | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37400 | - |
2 * 37400, recombinant His-tagged enzyme, SDS-PAGE | Helicobacter pylori |
| 75000 | - |
about, recombinant His-tagged enzyme, gel filtration | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetylglucosamine | Helicobacter pylori | - |
UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
strains 26695 and J99 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged FlaA1 9.9fold from Escherichia coli strain BL21(DE3) to homogeneity by nickel chelation and cation exchange chromatography | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | FlaA1 is a bifunctional C6 dehydratase/C4 reductase specific for UDPGlcNAc. It converts UDP-GlcNAc into a UDP-4-keto-6-methyl-GlcNAc intermediate, which is stereospecifically reduced into UDP-QuiNAc | Helicobacter pylori | ? | - |
? | |
| UDP-N-acetylglucosamine | - |
Helicobacter pylori | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 37400, recombinant His-tagged enzyme, SDS-PAGE | Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FlaA1 | - |
Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Helicobacter pylori |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 65 | - |
Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Helicobacter pylori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Helicobacter pylori | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Helicobacter pylori | sequence calculation | - |
8.65 |
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