Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
2 * 95000, SDS-PAGE | Polyporus obtusus |
200000 | - |
gel filtration | Polyporus obtusus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Polyporus obtusus | - |
- |
- |
Polyporus obtusus AU124PD | - |
- |
- |
Purification (Comment) | Organism |
---|---|
precipitation with 20% (w/v) PEG 4000, DEAE-Sepharose column chromatography, and carboxymethyl-Sepharose column chromatography | Polyporus obtusus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Polyporus obtusus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
cleared lysate, at pH 6.0 and 25°C | Polyporus obtusus |
9 | - |
after 450fold purification, at pH 6.0 and 25°C | Polyporus obtusus |
Storage Stability | Organism |
---|---|
4°C, purified enzyme in 0.1 M sodium phosphate buffer (pH 7.0), several months, the enzyme remains stable | Polyporus obtusus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosone | i.e. D-arabino-hexos-2-ulose. The enzyme dehydrates D-arabino-hexos-2-ulose, generating a double bond between C-3 and C-4. This unsaturated compound is proposed to be the steady-state intermediate characterized by the spectral peak at 265 nm. This intermediate is chemically unstable and rearranges from its strained 1,5 ring form to a more stable 2,6 pyranose structure. Then the enzyme acts a second time, dehydrating this pyranose structure to form cortalcerone | Polyporus obtusus | cortalcerone + H2O | i.e. 5,6-dihydro-6-hydroxy-5-oxo-2H-pyran-6-carboxaldehyde | ? | |
D-glucosone | i.e. D-arabino-hexos-2-ulose. The enzyme dehydrates D-arabino-hexos-2-ulose, generating a double bond between C-3 and C-4. This unsaturated compound is proposed to be the steady-state intermediate characterized by the spectral peak at 265 nm. This intermediate is chemically unstable and rearranges from its strained 1,5 ring form to a more stable 2,6 pyranose structure. Then the enzyme acts a second time, dehydrating this pyranose structure to form cortalcerone | Polyporus obtusus AU124PD | cortalcerone + H2O | i.e. 5,6-dihydro-6-hydroxy-5-oxo-2H-pyran-6-carboxaldehyde | ? | |
D-xylosone | i.e. D-threo-pentos-2-ulose | Polyporus obtusus | ? | the product absorbs at 260 nm but no conversion into a 230 nm-absorbing product occurs. Because of its strained nature, the intermediate still rearranges, losing UV absorbance, but since it lacks the 6th carbon, it cannot form a 2,6-pyranose ring to generate a second substrate for the enzyme | ? | |
D-xylosone | i.e. D-threo-pentos-2-ulose | Polyporus obtusus AU124PD | ? | the product absorbs at 260 nm but no conversion into a 230 nm-absorbing product occurs. Because of its strained nature, the intermediate still rearranges, losing UV absorbance, but since it lacks the 6th carbon, it cannot form a 2,6-pyranose ring to generate a second substrate for the enzyme | ? | |
additional information | no activity with fructose | Polyporus obtusus | ? | - |
? | |
additional information | no activity with fructose | Polyporus obtusus AU124PD | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 95000, SDS-PAGE | Polyporus obtusus |
Synonyms | Comment | Organism |
---|---|---|
aldosulose-utilizing enzyme | - |
Polyporus obtusus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Polyporus obtusus |