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Literature summary for 4.2.1.11 extracted from

  • Cayir, E.; Erdemir, A.; Ozkan, E.; Topuzogullari, M.; Bolat, Z.B.; Akat, A.; Turgut-Balik, D.
    Cloning of intron-removed enolase gene and expression, purification, kinetic characterization of the enzyme from Theileria annulata (2014), Mol. Biotechnol., 56, 689-696.
    View publication on PubMed
Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Theileria annulata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.106
-
 2-phospho-D-glycerate 1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C Theileria annulata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Theileria annulata
93000
-
 gel filtration Theileria annulata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Theileria annulata
-
 phosphoenolpyruvate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Theileria annulata E9NJD0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Theileria annulata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Theileria annulata phosphoenolpyruvate + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 48000, SDS-PAGE Theileria annulata

Synonyms

Synonyms Comment Organism
enolase
-
 Theileria annulata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37
-
 2-phospho-D-glycerate 1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C Theileria annulata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
350
-
 2-phospho-D-glycerate 1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C Theileria annulata