Literature summary for 4.2.1.11 extracted from

Quinones, W.; Pena, P.; Domingo-Sananes, M.; Caceres, A.; Michels, P.A.; Avilan, L.; Concepcion, J.L.
Leishmania mexicana: molecular cloning and characterization of enolase (2007), Exp. Parasitol., 116, 241-251.

Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
isolated from a genomic library, over-expressed in Escherichia coli, expression vector pET28a	Leishmania mexicana

Inhibitors

Inhibitors	Comment	Organism	Structure
diphosphate	inhibits natural enolase and recombinant protein	Leishmania mexicana
Mg2+	above 2 mM	Leishmania mexicana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	2-phospho-D-glycerate	recombinant protein	Leishmania mexicana
0.08	-	2-phospho-D-glycerate	natural enolase	Leishmania mexicana
0.2	-	phosphoenolpyruvate	recombinant protein	Leishmania mexicana
0.21	-	phosphoenolpyruvate	natural enolase	Leishmania mexicana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	enzyme activity only in cytosolic fraction, inactive form detected in the microsomal fraction, membrane-association and localization at the external face of the plasma membrane determined by digitonin treatment and immunofluorescence studies	Leishmania mexicana	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activity depends on, optimal concentration for activity between 1 mM and 2 mM	Leishmania mexicana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	SDS-PAGE	Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Leishmania mexicana	-	phosphoenolpyruvate + H2O	-	r
2-phospho-D-glycerate	Leishmania mexicana AZV	-	phosphoenolpyruvate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Leishmania mexicana	Q3HL75	-	-
Leishmania mexicana AZV	Q3HL75	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein and natural enolase	Leishmania mexicana

Source Tissue

Source Tissue	Comment	Organism	Textmining
promastigote	-	Leishmania mexicana	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	enolase activity measured spectrophotometrically by following the change in phosphoenolpyruvate concentration, substrate concentrations up to 0.3 mM for 2-phospho-D-glycerate and up to 2 mM for phosphoenolpyruvate	Leishmania mexicana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Leishmania mexicana	phosphoenolpyruvate + H2O	-	r
2-phospho-D-glycerate	-	Leishmania mexicana AZV	phosphoenolpyruvate + H2O	-	r

Synonyms

Synonyms	Comment	Organism
enolase	-	Leishmania mexicana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	activity assay at	Leishmania mexicana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	activity assay at	Leishmania mexicana

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.127	-	diphosphate	recombinant protein, substrate 2-phospho-D-glycerate	Leishmania mexicana
0.232	-	diphosphate	natural enolase, substrate 2-phospho-D-glycerate	Leishmania mexicana
0.238	-	diphosphate	natural enolase, substrate phosphoenolpyruvate	Leishmania mexicana
0.26	-	diphosphate	recombinant protein, substrate 2-phospho-D-glycerate	Leishmania mexicana

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Release 2023.1 (January 2023)