Application | Comment | Organism |
---|---|---|
molecular biology | alpha-enolase doubles as a surface-displayed plasminogen-binder supporting virulence | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
2.0 A resolution, molecular replacement, hanging-drop vapor-diffusion | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
deltaK433/K434 | mutant with different oligomerization state | Streptococcus pneumoniae |
K433L/K434L | mutant with different oligomerization state | Streptococcus pneumoniae |
K434L | mutant with different oligomerization state | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
370000 | - |
gel filtration | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Streptococcus pneumoniae | - |
phosphoenolpyruvate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q97QS2 | - |
- |
Purification (Comment) | Organism |
---|---|
ion exchange chromatography and gel filtration | Streptococcus pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
111 | - |
room temperature, pH 7.5, MgCl2, KCl, 2-phosphoglyceric acid | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Streptococcus pneumoniae | phosphoenolpyruvate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | - |
Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
plasminogen-binding alpha-enolase | - |
Streptococcus pneumoniae |