C107A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C107A/C117A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C117A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C71A
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme
Nostoc punctiforme
C71A/C107A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71A/C107A/C117A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71A/C117A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71S
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme
Nostoc punctiforme
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme
an alkane + formate + H2O + 2 NADP+
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
an alkane + formate + H2O + 2 NADP+
?
additional information
GC-MS measurements and identification of products
749051
Nostoc punctiforme
?
?
additional information
GC-MS measurements and identification of products
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
?
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme
pentadecane + formate + H2O + 2 NADP+
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
pentadecane + formate + H2O + 2 NADP+
?
n-octadecanal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme
heptadecane + formate + H2O + 2 NADP+
?
n-octadecanal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
heptadecane + formate + H2O + 2 NADP+
?
n-octadecenal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme
1-heptadecene + formate + H2O + 2 NADP+
?
n-octadecenal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
1-heptadecene + formate + H2O + 2 NADP+
?
C107A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C107A/C117A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C117A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C71A
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme
Nostoc punctiforme
C71A/C107A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71A/C107A/C117A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71A/C117A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71S
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme
Nostoc punctiforme
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme
an alkane + formate + H2O + 2 NADP+
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
an alkane + formate + H2O + 2 NADP+
?
additional information
GC-MS measurements and identification of products
749051
Nostoc punctiforme
?
?
additional information
GC-MS measurements and identification of products
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
?
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme
pentadecane + formate + H2O + 2 NADP+
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
pentadecane + formate + H2O + 2 NADP+
?
n-octadecanal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme
heptadecane + formate + H2O + 2 NADP+
?
n-octadecanal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
heptadecane + formate + H2O + 2 NADP+
?
n-octadecenal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme
1-heptadecene + formate + H2O + 2 NADP+
?
n-octadecenal + O2 + 2 NADPH + 2 H+
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
1-heptadecene + formate + H2O + 2 NADP+
?
747409
Wang
Identification of residues im ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechocystis sp. PCC 6803, Synechococcus elongatus PCC 7942 = FACHB-805 R2
BMC Biotechnol.
17
31-39
2017
-
-
-
-
21
-
-
9
-
2
-
3
-
13
-
-
-
-
-
-
-
-
11
1
7
-
-
-
29
-
-
-
1
-
-
-
-
-
-
1
-
21
-
-
-
-
9
-
2
-
3
-
-
-
-
-
-
-
-
11
1
-
-
-
29
-
-
-
-
-
2
2
-
9
9
748564
Patrikainen
Comparison of orthologous cya ...
Synechococcus sp. RS9917, Nostoc punctiforme, Synechocystis sp. PCC 6803, Prochlorococcus marinus, Nostoc punctiforme ATCC 29133 / PCC 73102, Prochlorococcus marinus MIT 9313
Metab. Eng. Commun.
5
9-18
2017
-
-
4
-
5
-
-
-
-
-
-
6
-
13
-
-
-
-
-
-
-
-
30
-
14
-
-
-
-
-
-
-
4
-
-
-
-
-
4
4
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746976
Park
Crystal structures of aldehyd ...
Oscillatoria sp. KNUA011, Limnothrix redekei, Limnothrix redekei KNUA012
Biochem. Biophys. Res. Commun.
477
395-400
2016
-
2
2
2
-
-
-
-
-
-
-
3
-
7
-
-
-
-
-
-
-
-
6
2
4
2
-
-
-
2
-
-
2
-
-
-
-
2
2
2
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
4
4
-
-
-
747351
Bao
Structure-oriented substrate ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechococcus elongatus PCC 7942 = FACHB-805 R2
Biotechnol. Biofuels
9
185
2016
-
-
1
-
13
-
-
7
-
-
-
2
-
7
-
-
1
-
-
-
-
-
14
-
6
1
-
-
8
1
-
-
1
-
-
-
-
-
1
1
-
13
-
-
-
-
7
-
-
-
2
-
-
-
1
-
-
-
-
14
-
1
-
-
8
1
-
-
-
-
2
2
-
8
8
747353
Zhang
Microbial synthesis of propan ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
Biotechnol. Biofuels
9
80
2016
-
-
1
-
3
-
-
-
-
-
-
2
-
8
-
-
-
-
-
-
-
-
4
-
3
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
747081
Warui
Efficient delivery of long-ch ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
Biochemistry
54
1006-1015
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
11
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748027
Rajakovich
Rapid reduction of the diferr ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
J. Am. Chem. Soc.
137
11695-11709
2015
-
-
-
-
-
-
-
-
-
1
-
2
-
9
-
-
-
1
-
-
-
-
10
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
748032
Shokri
Conversion of aldehyde to alk ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
J. Am. Chem. Soc.
137
7686-7691
2015
-
1
-
-
-
-
-
-
-
1
-
2
-
7
-
-
-
1
-
-
-
-
8
1
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749051
Hayashi
Role of cysteine residues in ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
PLoS ONE
10
e0122217
2015
-
-
1
-
8
-
-
1
-
1
-
2
-
8
-
-
1
-
-
-
-
-
10
-
2
1
-
5
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
10
-
1
-
5
-
-
-
-
-
-
3
3
-
-
-
749212
Jia
Structural insights into the ...
Synechococcus elongatus PCC 7942 = FACHB-805, Synechococcus elongatus PCC 7942 = FACHB-805 R2
Protein Cell
6
55-67
2015
-
-
1
1
3
-
-
-
-
1
-
2
-
6
-
-
1
1
-
-
-
-
6
1
5
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
726552
Das
Mechanistic insights from reac ...
Nostoc punctiforme
ACS Chem. Biol.
9
570-577
2014
-
-
1
-
-
-
1
-
-
1
-
1
-
3
-
-
1
1
-
-
-
-
6
-
3
1
-
-
3
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
6
-
1
-
-
3
1
-
-
-
-
1
1
-
-
-
746597
Buer
Insights into substrate and m ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Chem. Biol.
9
2584-2593
2014
-
-
-
1
1
-
1
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
-
4
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
747074
Waugh
Solvent isotope effects on al ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
Biochemistry
53
5537-5543
2014
-
-
1
-
-
-
-
1
-
1
-
2
1
3
-
-
1
1
-
-
-
-
6
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
1
-
2
1
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
726548
Aukema
Cyanobacterial aldehyde deform ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Catal.
3
2228-2238
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
8
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727215
Zhang
Conversion of fatty aldehydes ...
Synechococcus elongatus, Synechococcus elongatus PCC 7942
Biotechnol. Biofuels
6
86
2013
-
-
1
-
-
-
-
1
-
-
1
2
-
5
-
-
1
-
-
-
-
-
10
1
3
1
-
-
1
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
-
-
10
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
727312
Khara
Production of propane and othe ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
ChemBioChem
14
1204-1208
2013
-
-
1
-
4
-
-
-
-
1
-
2
-
3
-
-
1
1
-
-
-
-
8
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
727690
Pandelia
Substrate-triggered addition o ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
15801-15812
2013
1
-
1
-
-
-
-
1
-
1
-
1
-
3
-
-
1
1
-
-
-
-
2
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
727693
Paul
Probing the mechanism of cyano ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
5234-5237
2013
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727001
Li
Evidence for only oxygenative ...
Prochlorococcus marinus
Biochemistry
51
7908-7916
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
715293
Warui
Detection of formate, rather t ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
3316-3319
2011
-
-
2
-
-
-
-
-
-
1
-
2
-
5
-
-
1
1
-
-
-
-
4
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
715300
Li
Conversion of fatty aldehydes ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
6158-6161
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726957
Eser
Oxygen-independent alkane form ...
Synechocystis sp., Nostoc punctiforme, Prochlorococcus marinus, Synechococcus sp., Prochlorococcus marinus MIT9313
Biochemistry
50
10743-10750
2011
-
-
4
-
-
-
4
-
-
-
-
-
-
9
-
-
-
4
-
-
-
-
15
-
8
4
-
-
-
4
-
-
4
-
-
-
-
-
4
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
4
-
-
-
4
-
-
-
-
4
4
-
-
-
713511
Schirmer
Microbial biosynthesis of alka ...
Nostoc punctiforme
Science
329
559-562
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
648395
Schneider-Belhaddad
Solubilization, partial purifi ...
Pisum sativum
Arch. Biochem. Biophys.
377
341-349
2000
4
-
-
-
-
-
7
-
2
1
1
1
-
8
1
-
1
-
-
1
1
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
9
-
-
2
1
1
1
-
1
-
1
-
1
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648396
Kobayashi
Cobalt proteins ...
Botryococcus braunii
Eur. J. Biochem.
261
1-9
1999
-
-
-
-
-
-
-
-
-
1
2
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648394
Dennis
A cobalt-porphyrin enzyme conv ...
Botryococcus braunii, Botryococcus braunii Austin
Proc. Natl. Acad. Sci. USA
89
5306-5310
1992
-
-
-
-
-
-
1
-
1
1
2
2
-
6
-
-
1
-
-
-
1
-
4
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
2
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648393
Dennis
Alkane biosynthesis by decarbo ...
Botryococcus braunii, Botryococcus braunii Austin
Arch. Biochem. Biophys.
287
268-275
1991
1
-
-
-
-
-
5
1
2
1
-
2
-
6
1
-
-
-
-
-
-
-
4
-
-
1
-
1
-
1
1
-
1
-
-
-
1
-
-
1
-
-
-
-
5
-
1
2
1
-
2
-
1
-
-
-
-
-
-
4
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
648392
Cheesbrough
Microsomal preparation from an ...
Podiceps nigricollis
J. Biol. Chem.
263
2738-2743
1988
2
-
-
-
-
-
9
1
2
1
-
1
-
3
1
-
-
-
-
1
-
-
2
-
-
1
-
1
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
9
-
1
2
1
-
1
-
1
-
-
-
1
-
-
2
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
648391
Cheesbrough
Alkane biosynthesis by decarbo ...
Pisum sativum
Proc. Natl. Acad. Sci. USA
81
6613-6617
1984
-
-
-
-
-
2
4
1
1
1
-
1
-
4
-
-
-
-
-
2
-
-
2
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
2
-
6
-
1
1
1
-
1
-
-
-
-
-
2
-
-
2
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-