BRENDA - Enzyme Database show
show all sequences of 4.1.99.5

Role of cysteine residues in the structure, stability, and alkane producing activity of cyanobacterial aldehyde deformylating oxygenase

Hayashi, Y.; Yasugi, F.; Arai, M.; PLoS ONE 10, e0122217 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
Nostoc punctiforme
Engineering
Amino acid exchange
Commentary
Organism
C107A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C107A/C117A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C117A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C71A
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme
Nostoc punctiforme
C71A/C107A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71A/C107A/C117A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71A/C117A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71S
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme
Nostoc punctiforme
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Nostoc punctiforme
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
di-iron center, the amount of hydrocarbons produced in recombinant Escherichia coli is decreased when the iron concentration in the M9 medium is decreased. The Cys-to-Ala/Ser mutations do not affect the iron binding to the enzyme
Nostoc punctiforme
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Nostoc punctiforme
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Nostoc punctiforme ATCC 29133 / PCC 73102
-
an alkane + formate + H2O + 2 NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nostoc punctiforme
B2J1M1
-
-
Nostoc punctiforme ATCC 29133 / PCC 73102
B2J1M1
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis
Nostoc punctiforme
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
an alkane + formate + H2O + 2 NADP+
-
-
-
?
additional information
GC-MS measurements and identification of products
749051
Nostoc punctiforme
?
-
-
-
-
additional information
GC-MS measurements and identification of products
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
?
-
-
-
-
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
n-octadecanal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme
n-heptadecane + formate + H2O + 2 NADP+
-
-
-
?
n-octadecanal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
n-heptadecane + formate + H2O + 2 NADP+
-
-
-
?
n-octadecenal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme
n-heptadecene + formate + H2O + 2 NADP+
-
-
-
?
n-octadecenal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
n-heptadecene + formate + H2O + 2 NADP+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
in vivo assay at
Nostoc punctiforme
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
43
-
Tm of recombinant mutant C107A/C117A
Nostoc punctiforme
46
-
Tm of recombinant mutant C71S
Nostoc punctiforme
47
-
Tm of recombinant mutants C71A, C107A, C117A, and C71A/C117A
Nostoc punctiforme
48
-
Tm of recombinant mutant C71A/C107A
Nostoc punctiforme
53
-
Tm of recombinant wild-type
Nostoc punctiforme
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Nostoc punctiforme
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
Nostoc punctiforme
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Nostoc punctiforme
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C107A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C107A/C117A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C117A
site-directed mutagenesis, the mutation does not affect the hydrocarbon producing activity of the enzyme
Nostoc punctiforme
C71A
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme
Nostoc punctiforme
C71A/C107A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71A/C107A/C117A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71A/C117A
site-directed mutagenesis, the mutant has reduced activity compared to wild-type, and an activity comparable to or even lower than the activity of the C71A variant
Nostoc punctiforme
C71S
site-directed mutagenesis, the mutant shows reduced hydrocarbon producing activity and facilitated formation of a dimer compared to wild-type enzyme
Nostoc punctiforme
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Nostoc punctiforme
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
di-iron center, the amount of hydrocarbons produced in recombinant Escherichia coli is decreased when the iron concentration in the M9 medium is decreased. The Cys-to-Ala/Ser mutations do not affect the iron binding to the enzyme
Nostoc punctiforme
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Nostoc punctiforme
-
an alkane + formate + H2O + 2 NADP+
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
Nostoc punctiforme ATCC 29133 / PCC 73102
-
an alkane + formate + H2O + 2 NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis
Nostoc punctiforme
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme
an alkane + formate + H2O + 2 NADP+
-
-
-
?
a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
an alkane + formate + H2O + 2 NADP+
-
-
-
?
additional information
GC-MS measurements and identification of products
749051
Nostoc punctiforme
?
-
-
-
-
additional information
GC-MS measurements and identification of products
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
?
-
-
-
-
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
n-hexadecanal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
n-pentadecane + formate + H2O + 2 NADP+
-
-
-
?
n-octadecanal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme
n-heptadecane + formate + H2O + 2 NADP+
-
-
-
?
n-octadecanal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
n-heptadecane + formate + H2O + 2 NADP+
-
-
-
?
n-octadecenal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme
n-heptadecene + formate + H2O + 2 NADP+
-
-
-
?
n-octadecenal + O2 + 2 NADPH + 2 H+
-
749051
Nostoc punctiforme ATCC 29133 / PCC 73102
n-heptadecene + formate + H2O + 2 NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
in vivo assay at
Nostoc punctiforme
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
43
-
Tm of recombinant mutant C107A/C117A
Nostoc punctiforme
46
-
Tm of recombinant mutant C71S
Nostoc punctiforme
47
-
Tm of recombinant mutants C71A, C107A, C117A, and C71A/C117A
Nostoc punctiforme
48
-
Tm of recombinant mutant C71A/C107A
Nostoc punctiforme
53
-
Tm of recombinant wild-type
Nostoc punctiforme
General Information
General Information
Commentary
Organism
malfunction
C71A/S mutations reduce the hydrocarbon producing activity of AD and facilitate the formation of a dimer, while mutations at Cys107 and Cys117 do not affect the hydrocarbon producing activity of the enzyme. The Cys-to-Ala/Ser mutations do not affect the iron binding to the enzyme. Structural features of the Cys-deficient mutants, overview
Nostoc punctiforme
additional information
Cys71, which is located in close proximity to the substrate-binding site, plays a crucial role in maintaining the activity, structure, and stability of the enzyme
Nostoc punctiforme
physiological function
aldehyde deformylating oxygenase is a key enzyme for alkane biosynthesis in cyanobacteria
Nostoc punctiforme
General Information (protein specific)
General Information
Commentary
Organism
malfunction
C71A/S mutations reduce the hydrocarbon producing activity of AD and facilitate the formation of a dimer, while mutations at Cys107 and Cys117 do not affect the hydrocarbon producing activity of the enzyme. The Cys-to-Ala/Ser mutations do not affect the iron binding to the enzyme. Structural features of the Cys-deficient mutants, overview
Nostoc punctiforme
additional information
Cys71, which is located in close proximity to the substrate-binding site, plays a crucial role in maintaining the activity, structure, and stability of the enzyme
Nostoc punctiforme
physiological function
aldehyde deformylating oxygenase is a key enzyme for alkane biosynthesis in cyanobacteria
Nostoc punctiforme
Other publictions for EC 4.1.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747409
Wang
Identification of residues im ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2, Synechocystis sp. PCC 6803
BMC Biotechnol.
17
31-39
2017
-
-
-
-
21
-
-
8
-
2
-
3
-
9
-
-
-
-
-
-
-
-
11
1
-
-
-
28
-
-
-
1
-
-
-
-
-
-
1
-
21
-
-
-
-
8
-
2
-
3
-
-
-
-
-
-
-
-
11
1
-
-
-
28
-
-
-
-
-
2
2
-
9
9
748564
Patrikainen
Comparison of orthologous cya ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102, Prochlorococcus marinus, Prochlorococcus marinus MIT 9313, Synechococcus sp. RS9917, Synechocystis sp. PCC 6803
Metab. Eng. Commun.
5
9-18
2017
-
-
4
-
5
-
-
-
-
-
-
6
-
13
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
4
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746976
Park
Crystal structures of aldehyd ...
Limnothrix redekei, Limnothrix redekei KNUA012, Oscillatoria sp. KNUA011
Biochem. Biophys. Res. Commun.
477
395-400
2016
-
2
2
2
-
-
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
2
-
-
-
-
2
2
2
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
4
4
-
-
-
747351
Bao
Structure-oriented substrate ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2
Biotechnol. Biofuels
9
185
2016
-
-
1
-
13
-
-
7
-
-
-
2
-
6
-
-
1
-
-
-
-
-
14
-
1
-
-
8
1
-
-
1
-
-
-
-
-
1
1
-
13
-
-
-
-
7
-
-
-
2
-
-
-
1
-
-
-
-
14
-
1
-
-
8
1
-
-
-
-
2
2
-
8
8
747353
Zhang
Microbial synthesis of propan ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
Biotechnol. Biofuels
9
80
2016
-
-
1
-
3
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
747081
Warui
Efficient delivery of long-ch ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
Biochemistry
54
1006-1015
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748027
Rajakovich
Rapid reduction of the diferr ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
J. Am. Chem. Soc.
137
11695-11709
2015
-
-
-
-
-
-
-
-
-
1
-
2
-
8
-
-
-
1
-
-
-
-
10
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
10
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
748032
Shokri
Conversion of aldehyde to alk ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
J. Am. Chem. Soc.
137
7686-7691
2015
-
1
-
-
-
-
-
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749051
Hayashi
Role of cysteine residues in ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
PLoS ONE
10
e0122217
2015
-
-
1
-
8
-
-
1
-
1
-
2
-
7
-
-
1
-
-
-
-
-
10
-
1
-
5
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
10
-
1
-
5
-
-
-
-
-
-
3
3
-
-
-
749212
Jia
Structural insights into the ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2
Protein Cell
6
55-67
2015
-
-
1
1
3
-
-
-
-
1
-
2
-
6
-
-
1
1
-
-
-
-
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
726552
Das
Mechanistic insights from reac ...
Nostoc punctiforme
ACS Chem. Biol.
9
570-577
2014
-
-
1
-
-
-
1
-
-
1
-
1
-
2
-
-
1
1
-
-
-
-
6
-
1
-
-
3
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
6
-
1
-
-
3
1
-
-
-
-
1
1
-
-
-
746597
Buer
Insights into substrate and m ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Chem. Biol.
9
2584-2593
2014
-
-
-
1
1
-
1
-
-
1
-
2
-
6
-
-
-
1
-
-
-
-
8
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
1
1
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1
-
2
-
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-
-
-
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-
-
8
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
747074
Waugh
Solvent isotope effects on al ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
Biochemistry
53
5537-5543
2014
-
-
1
-
-
-
-
1
-
1
-
2
1
3
-
-
1
1
-
-
-
-
6
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
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1
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-
2
1
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
726548
Aukema
Cyanobacterial aldehyde deform ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Catal.
3
2228-2238
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727215
Zhang
Conversion of fatty aldehydes ...
Synechococcus elongatus
Biotechnol. Biofuels
6
86
2013
-
-
1
-
-
-
-
1
-
-
1
1
-
3
-
-
1
-
-
-
-
-
5
1
1
-
-
1
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
-
-
5
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
727312
Khara
Production of propane and othe ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
ChemBioChem
14
1204-1208
2013
-
-
1
-
4
-
-
-
-
1
-
2
-
3
-
-
1
1
-
-
-
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
727690
Pandelia
Substrate-triggered addition o ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
15801-15812
2013
1
-
1
-
-
-
-
1
-
1
-
1
-
2
-
-
1
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
727693
Paul
Probing the mechanism of cyano ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
5234-5237
2013
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
727001
Li
Evidence for only oxygenative ...
Prochlorococcus marinus
Biochemistry
51
7908-7916
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
715293
Warui
Detection of formate, rather t ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
3316-3319
2011
-
-
1
-
-
-
-
-
-
1
-
2
-
4
-
-
1
1
-
-
-
-
4
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
715300
Li
Conversion of fatty aldehydes ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
6158-6161
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726957
Eser
Oxygen-independent alkane form ...
Nostoc punctiforme, Prochlorococcus marinus, Prochlorococcus marinus MIT9313, Synechococcus sp., Synechocystis sp.
Biochemistry
50
10743-10750
2011
-
-
4
-
-
-
4
-
-
-
-
-
-
8
-
-
-
4
-
-
-
-
15
-
4
-
-
-
4
-
-
4
-
-
-
-
-
4
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
4
-
-
-
4
-
-
-
-
4
4
-
-
-
713511
Schirmer
Microbial biosynthesis of alka ...
Nostoc punctiforme
Science
329
559-562
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
648395
Schneider-Belhaddad
Solubilization, partial purifi ...
Pisum sativum
Arch. Biochem. Biophys.
377
341-349
2000
4
-
-
-
-
-
7
-
2
1
1
1
-
4
1
-
1
-
-
1
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
7
-
-
2
1
1
1
-
1
-
1
-
1
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648396
Kobayashi
Cobalt proteins ...
Botryococcus braunii
Eur. J. Biochem.
261
1-9
1999
-
-
-
-
-
-
-
-
-
1
2
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
648394
Dennis
A cobalt-porphyrin enzyme conv ...
Botryococcus braunii, Botryococcus braunii Austin
Proc. Natl. Acad. Sci. USA
89
5306-5310
1992
-
-
-
-
-
-
1
-
1
1
2
2
-
5
-
-
1
-
-
-
1
-
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
2
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
648393
Dennis
Alkane biosynthesis by decarbo ...
Botryococcus braunii, Botryococcus braunii Austin
Arch. Biochem. Biophys.
287
268-275
1991
1
-
-
-
-
-
5
1
2
1
-
2
-
6
1
-
-
-
-
-
-
-
4
-
1
-
1
-
1
1
-
1
-
-
-
1
-
-
1
-
-
-
-
5
-
1
2
1
-
2
-
1
-
-
-
-
-
-
4
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
648392
Cheesbrough
Microsomal preparation from an ...
Podiceps nigricollis
J. Biol. Chem.
263
2738-2743
1988
2
-
-
-
-
-
9
1
2
1
-
1
-
3
1
-
-
-
-
1
-
-
2
-
1
-
1
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
9
-
1
2
1
-
1
-
1
-
-
-
1
-
-
2
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
648391
Cheesbrough
Alkane biosynthesis by decarbo ...
Pisum sativum
Proc. Natl. Acad. Sci. USA
81
6613-6617
1984
-
-
-
-
-
2
4
1
1
1
-
1
-
3
-
-
-
-
-
2
-
-
2
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
2
-
4
-
1
1
1
-
1
-
-
-
-
-
2
-
-
2
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-