BRENDA - Enzyme Database show
show all sequences of 4.1.99.5

Cyanobacterial aldehyde deformylase oxygenation of aldehydes yields n-1 aldehydes and alcohols in addition to alkanes

Aukema, K.G.; Makris, T.M.; Stoian, S.A.; Richman, J.E.; Muenck, E.; Lipscomb, J.D.; Wackett, L.P.; ACS Catal. 3, 2228-2238 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Prochlorococcus marinus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Prochlorococcus marinus
Q7V6D4
gene PMT_1231
-
Prochlorococcus marinus MIT 9313
Q7V6D4
gene PMT_1231
-
Purification (Commentary)
Commentary
Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and dialysis
Prochlorococcus marinus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
decanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus
nonane + formate + H2O + 2 NAD+
-
-
-
?
decanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus MIT 9313
nonane + formate + H2O + 2 NAD+
-
-
-
?
additional information
substrate specificity, overview. Marked decrease in relative yields of aldehyde and alcohol products are observed as the alkyl chain length is decreased from C9 to C8. The relative yields of the one-carbon-shorter alcohol and aldehyde products are optimal with nonanal and decanal and decrease with shorter and longer alkyl chains
726548
Prochlorococcus marinus
?
-
-
-
-
additional information
substrate specificity, overview. Marked decrease in relative yields of aldehyde and alcohol products are observed as the alkyl chain length is decreased from C9 to C8. The relative yields of the one-carbon-shorter alcohol and aldehyde products are optimal with nonanal and decanal and decrease with shorter and longer alkyl chains
726548
Prochlorococcus marinus MIT 9313
?
-
-
-
-
nonanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus
octane + formate + H2O + 2 NAD+
-
-
-
?
nonanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus MIT 9313
octane + formate + H2O + 2 NAD+
-
-
-
?
octadecanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus
heptadecane + formate + H2O + 2 NAD+
-
-
-
?
octadecanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus MIT 9313
heptadecane + formate + H2O + 2 NAD+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at room temperature
Prochlorococcus marinus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Prochlorococcus marinus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Prochlorococcus marinus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Prochlorococcus marinus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Prochlorococcus marinus
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and dialysis
Prochlorococcus marinus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
decanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus
nonane + formate + H2O + 2 NAD+
-
-
-
?
decanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus MIT 9313
nonane + formate + H2O + 2 NAD+
-
-
-
?
additional information
substrate specificity, overview. Marked decrease in relative yields of aldehyde and alcohol products are observed as the alkyl chain length is decreased from C9 to C8. The relative yields of the one-carbon-shorter alcohol and aldehyde products are optimal with nonanal and decanal and decrease with shorter and longer alkyl chains
726548
Prochlorococcus marinus
?
-
-
-
-
additional information
substrate specificity, overview. Marked decrease in relative yields of aldehyde and alcohol products are observed as the alkyl chain length is decreased from C9 to C8. The relative yields of the one-carbon-shorter alcohol and aldehyde products are optimal with nonanal and decanal and decrease with shorter and longer alkyl chains
726548
Prochlorococcus marinus MIT 9313
?
-
-
-
-
nonanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus
octane + formate + H2O + 2 NAD+
-
-
-
?
nonanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus MIT 9313
octane + formate + H2O + 2 NAD+
-
-
-
?
octadecanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus
heptadecane + formate + H2O + 2 NAD+
-
-
-
?
octadecanal + O2 + 2 NADH + 2 H+
with reducing system NADH/phenazine
726548
Prochlorococcus marinus MIT 9313
heptadecane + formate + H2O + 2 NAD+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at room temperature
Prochlorococcus marinus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Prochlorococcus marinus
Other publictions for EC 4.1.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747409
Wang
Identification of residues im ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2, Synechocystis sp. PCC 6803
BMC Biotechnol.
17
31-39
2017
-
-
-
-
21
-
-
8
-
2
-
3
-
9
-
-
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-
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-
11
1
-
-
-
28
-
-
-
1
-
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-
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-
-
1
-
21
-
-
-
-
8
-
2
-
3
-
-
-
-
-
-
-
-
11
1
-
-
-
28
-
-
-
-
-
2
2
-
9
9
748564
Patrikainen
Comparison of orthologous cya ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102, Prochlorococcus marinus, Prochlorococcus marinus MIT 9313, Synechococcus sp. RS9917, Synechocystis sp. PCC 6803
Metab. Eng. Commun.
5
9-18
2017
-
-
4
-
5
-
-
-
-
-
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6
-
13
-
-
-
-
-
-
-
-
30
-
-
-
-
-
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-
4
-
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4
4
-
5
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6
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-
30
-
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-
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-
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-
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-
-
-
-
-
746976
Park
Crystal structures of aldehyd ...
Limnothrix redekei, Limnothrix redekei KNUA012, Oscillatoria sp. KNUA011
Biochem. Biophys. Res. Commun.
477
395-400
2016
-
2
2
2
-
-
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-
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3
-
5
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6
2
2
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2
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2
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2
2
2
2
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3
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6
2
2
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2
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4
4
-
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747351
Bao
Structure-oriented substrate ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2
Biotechnol. Biofuels
9
185
2016
-
-
1
-
13
-
-
7
-
-
-
2
-
6
-
-
1
-
-
-
-
-
14
-
1
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-
8
1
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1
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-
1
1
-
13
-
-
-
-
7
-
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-
2
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-
-
1
-
-
-
-
14
-
1
-
-
8
1
-
-
-
-
2
2
-
8
8
747353
Zhang
Microbial synthesis of propan ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
Biotechnol. Biofuels
9
80
2016
-
-
1
-
3
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
1
-
-
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1
1
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3
-
-
-
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-
2
-
-
-
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-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
747081
Warui
Efficient delivery of long-ch ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
Biochemistry
54
1006-1015
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
-
-
-
2
-
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1
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1
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2
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-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748027
Rajakovich
Rapid reduction of the diferr ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
J. Am. Chem. Soc.
137
11695-11709
2015
-
-
-
-
-
-
-
-
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1
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2
-
8
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1
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10
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1
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1
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2
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1
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2
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-
10
-
1
-
-
-
1
-
-
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-
1
1
-
-
-
748032
Shokri
Conversion of aldehyde to alk ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
J. Am. Chem. Soc.
137
7686-7691
2015
-
1
-
-
-
-
-
-
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1
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2
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6
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1
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8
1
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1
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1
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1
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1
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2
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8
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
749051
Hayashi
Role of cysteine residues in ...
Nostoc punctiforme, Nostoc punctiforme ATCC 29133 / PCC 73102
PLoS ONE
10
e0122217
2015
-
-
1
-
8
-
-
1
-
1
-
2
-
7
-
-
1
-
-
-
-
-
10
-
1
-
5
-
-
-
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1
-
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1
1
-
8
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
10
-
1
-
5
-
-
-
-
-
-
3
3
-
-
-
749212
Jia
Structural insights into the ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 R2
Protein Cell
6
55-67
2015
-
-
1
1
3
-
-
-
-
1
-
2
-
6
-
-
1
1
-
-
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6
1
1
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1
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1
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1
1
1
3
-
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1
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2
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1
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-
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6
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
726552
Das
Mechanistic insights from reac ...
Nostoc punctiforme
ACS Chem. Biol.
9
570-577
2014
-
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1
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1
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1
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1
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2
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1
1
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6
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1
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3
1
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2
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1
2
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1
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1
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1
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1
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6
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1
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3
1
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1
1
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746597
Buer
Insights into substrate and m ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Chem. Biol.
9
2584-2593
2014
-
-
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1
1
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1
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1
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2
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6
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1
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8
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1
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1
1
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2
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8
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1
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2
2
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747074
Waugh
Solvent isotope effects on al ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
Biochemistry
53
5537-5543
2014
-
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1
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1
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1
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2
1
3
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1
1
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6
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1
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1
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1
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6
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1
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1
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1
1
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726548
Aukema
Cyanobacterial aldehyde deform ...
Prochlorococcus marinus, Prochlorococcus marinus MIT 9313
ACS Catal.
3
2228-2238
2013
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1
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6
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1
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8
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1
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1
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727215
Zhang
Conversion of fatty aldehydes ...
Synechococcus elongatus
Biotechnol. Biofuels
6
86
2013
-
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1
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1
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1
1
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3
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1
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5
1
1
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1
1
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727312
Khara
Production of propane and othe ...
Prochlorococcus marinus, Prochlorococcus marinus MIT9313
ChemBioChem
14
1204-1208
2013
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1
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4
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1
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2
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3
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1
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1
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4
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1
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8
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1
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1
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1
1
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727690
Pandelia
Substrate-triggered addition o ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
15801-15812
2013
1
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1
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1
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1
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1
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2
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1
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2
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1
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1
1
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727693
Paul
Probing the mechanism of cyano ...
Nostoc punctiforme
J. Am. Chem. Soc.
135
5234-5237
2013
-
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1
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1
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1
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3
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1
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1
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727001
Li
Evidence for only oxygenative ...
Prochlorococcus marinus
Biochemistry
51
7908-7916
2012
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1
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4
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3
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1
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1
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1
1
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715293
Warui
Detection of formate, rather t ...
Nostoc punctiforme
J. Am. Chem. Soc.
133
3316-3319
2011
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1
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1
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2
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4
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1
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1
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1
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1
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4
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1
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1
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1
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726957
Eser
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4
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4
4
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4
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15
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4
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4
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4
4
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713511
Schirmer
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1
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1
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2
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1
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4
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7
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2
1
1
1
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1
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1
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1
1
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2
1
1
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648396
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5
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1
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1
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9
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Cheesbrough
Alkane biosynthesis by decarbo ...
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1
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1
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