BRENDA - Enzyme Database
show all sequences of 4.1.99.16

Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase

Cane, D.E.; He, X.; Kobayashi, S.; Omura, S.; Ikeda, H.; J. Antibiot. 59, 471-479 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Streptomyces avermitilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000075
-
(2E,6E)-farnesyl diphosphate
pH 8.2, 30°C
Streptomyces avermitilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
10-20% of the activity with Mg2+
Streptomyces avermitilis
Cu2+
10-20% of the activity with Mg2+
Streptomyces avermitilis
Fe2+
50% of the activity with Mg2+
Streptomyces avermitilis
Mg2+
preferred divalent cation, maximum activity at 10 mM
Streptomyces avermitilis
Mn2+
10-20% of the activity with Mg2+
Streptomyces avermitilis
additional information
absolute requirement for a divalent cation. No activity with Ni2+ or Ca2+
Streptomyces avermitilis
Zn2+
50% of the activity with Mg2+
Streptomyces avermitilis
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces avermitilis
Q82L49
germacradienol/geosmin synthase
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2E,6E)-farnesyl diphosphate + H2O
overall reaction of germacradienol/geosmin synthase
680396
Streptomyces avermitilis
(4S,7R)-germacra-1(10)E,5E-diene-11-ol + (7S)-germacrene D + geosmin + ?
presence of Mg2+, synthesis of 66% (4S,7R)-germacra-1(10)E,5E-diene-11-ol, 24% (7S)-germacrene D, 8% geosmin, and 2% of a hydrocarbon, tentatively assigned the structure of octalin
-
-
?
additional information
nerolidyl diphosphate is excluded as an intermediate in the enzymatic formation of germacradienol, germacrene D, and geosmin
680396
Streptomyces avermitilis
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
geoA
-
Streptomyces avermitilis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0031
-
(2E,6E)-farnesyl diphosphate
pH 8.2, 30°C
Streptomyces avermitilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
9
-
Streptomyces avermitilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces avermitilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000075
-
(2E,6E)-farnesyl diphosphate
pH 8.2, 30°C
Streptomyces avermitilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
10-20% of the activity with Mg2+
Streptomyces avermitilis
Cu2+
10-20% of the activity with Mg2+
Streptomyces avermitilis
Fe2+
50% of the activity with Mg2+
Streptomyces avermitilis
Mg2+
preferred divalent cation, maximum activity at 10 mM
Streptomyces avermitilis
Mn2+
10-20% of the activity with Mg2+
Streptomyces avermitilis
additional information
absolute requirement for a divalent cation. No activity with Ni2+ or Ca2+
Streptomyces avermitilis
Zn2+
50% of the activity with Mg2+
Streptomyces avermitilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2E,6E)-farnesyl diphosphate + H2O
overall reaction of germacradienol/geosmin synthase
680396
Streptomyces avermitilis
(4S,7R)-germacra-1(10)E,5E-diene-11-ol + (7S)-germacrene D + geosmin + ?
presence of Mg2+, synthesis of 66% (4S,7R)-germacra-1(10)E,5E-diene-11-ol, 24% (7S)-germacrene D, 8% geosmin, and 2% of a hydrocarbon, tentatively assigned the structure of octalin
-
-
?
additional information
nerolidyl diphosphate is excluded as an intermediate in the enzymatic formation of germacradienol, germacrene D, and geosmin
680396
Streptomyces avermitilis
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0031
-
(2E,6E)-farnesyl diphosphate
pH 8.2, 30°C
Streptomyces avermitilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
9
-
Streptomyces avermitilis
General Information
General Information
Commentary
Organism
physiological function
mutants with a deleted geoA are unable to produce either germacradienol or geosmin
Streptomyces avermitilis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
mutants with a deleted geoA are unable to produce either germacradienol or geosmin
Streptomyces avermitilis
Other publictions for EC 4.1.99.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743387
Schifrin
A single terpene synthase is ...
Sorangium cellulosum, Sorangium cellulosum Soce56
Org. Biomol. Chem.
14
3385-3393
2016
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2
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747096
Harris
Structural studies of geosmin ...
Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471 / A3(2) / M145
Biochemistry
54
7142-7155
2015
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1
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747590
Zhou
-
Cloning and expression analys ...
Aphanizomenon gracile, Aphanizomenon gracile WH-1
Chin. J. Appl. Environ. Biol.
21
824-829
2015
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1
1
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728186
Giglio
Expression of the geosmin synt ...
Dolichospermum circinale, Dolichospermum circinale AWQC318
J. Phycol.
47
1338-1343
2011
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1
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1
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712642
Singh
Exploration of geosmin synthas ...
Streptomyces peucetius, Streptomyces peucetius ATCC 27952
J. Ind. Microbiol. Biotechnol.
36
1257-1265
2009
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6
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692761
Jiang
Geosmin biosynthesis. Mechanis ...
Streptomyces coelicolor
J. Am. Chem. Soc.
130
428-429
2008
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680396
Cane
Geosmin biosynthesis in Strept ...
Streptomyces avermitilis
J. Antibiot.
59
471-479
2006
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1
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1
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7
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1
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