Literature summary for 4.1.3.40 extracted from

Stadthagen, G.; Kordulakova, J.; Griffin, R.; Constant, P.; Bottova, I.; Barilone, N.; Gicquel, B.; Daffe, M.; Jackson, M.
p-Hydroxybenzoic acid synthesis in Mycobacterium tuberculosis (2005), J. Biol. Chem., 280, 40699-40706.

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Cloned(Commentary)

Cloned (Comment)	Organism
production of recombinant enzyme in Escherichia coli and Mycobacterium smegmatis	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
4-hydroxybenzoate	strong product inhibition	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00197	-	chorismate	pH 7.5, 37°C, recombinant enzyme produced in Mycobacterium smegmatis	Mycobacterium tuberculosis
0.0396	-	chorismate	pH 7.5, 37°C, recombinant enzyme produced in Escherichia coli	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme produced in Escherichia coli and Mycobacterium smegmatis	Mycobacterium tuberculosis

Storage Stability

Storage Stability	Organism
-20°C, in presence of 10% glycerol the purified recombinant enzyme is stable for at least 3 weeks	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chorismate	-	Mycobacterium tuberculosis	4-hydroxybenzoate + pyruvate	-	?

Synonyms

Synonyms	Comment	Organism
Rv2949c enzyme	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.102	-	chorismate	pH 7.5, 37°C, recombinant enzyme produced in Mycobacterium smegmatis	Mycobacterium tuberculosis
0.215	-	chorismate	pH 7.5, 37°C, recombinant enzyme produced in Escherichia coli	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.001	-	4-hydroxybenzoate	pH 7.5, 37°C	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)