Application | Comment | Organism |
---|---|---|
medicine | TrpE is a promising target for the design of novel anti-tuberculosis drugs | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
into the vector pET30a for expression in Escherichia coli BL21DE3 cells | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tryptophan | - |
Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62300 | - |
recombinant fusion protein, determined by SDS-PAGE | Mycobacterium tuberculosis |
62350 | - |
determined by mass spectrometry | Mycobacterium tuberculosis |
62370 | - |
calculated | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + ammonia | Mycobacterium tuberculosis | - |
anthranilate + pyruvate + H2O | - |
? | |
chorismate + ammonia | Mycobacterium tuberculosis H37Rv | - |
anthranilate + pyruvate + H2O | - |
? | |
chorismate + L-glutamine | Mycobacterium tuberculosis | - |
anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + L-glutamine | Mycobacterium tuberculosis H37Rv | - |
anthranilate + pyruvate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WFX3 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WFX3 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA His-binding resin, the 6xHis-tag is removed by digestion with enterokinase | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.16 | - |
crude supernatant | Mycobacterium tuberculosis |
4.44 | - |
purification step Ni-NTA column | Mycobacterium tuberculosis |
5.2 | - |
after concentration step | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + ammonia | - |
Mycobacterium tuberculosis | anthranilate + pyruvate + H2O | - |
? | |
chorismate + ammonia | - |
Mycobacterium tuberculosis H37Rv | anthranilate + pyruvate + H2O | - |
? | |
chorismate + L-glutamine | - |
Mycobacterium tuberculosis | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + L-glutamine | - |
Mycobacterium tuberculosis H37Rv | anthranilate + pyruvate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
anthranilate synthase component I | - |
Mycobacterium tuberculosis |
TrpE | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | incubation at temperatures higher than 37°C results in a significant reduction of activity | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
activity assay | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7 20% relative activity, pH 10 60% relative activity | Mycobacterium tuberculosis |