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Literature summary for 4.1.2.62 extracted from

  • Beaudoin, G.A.W.; Li, Q.; Folz, J.; Fiehn, O.; Goodsell, J.L.; Angerhofer, A.; Bruner, S.D.; Hanson, A.D.
    Salvage of the 5-deoxyribose byproduct of radical SAM enzymes (2018), Nat. Commun., 9, 3105 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 CodonPlus (DE3)-RIPL cells Bacillus thuringiensis serovar kurstaki

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 200 mM NaCl, 28% (w/v) PEG-3350 and 100 mM HEPES-KOH, pH 7.5 Bacillus thuringiensis serovar kurstaki

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 5-deoxy-D-ribulose 1-phosphate at pH 8.2 and 21°C Bacillus thuringiensis serovar kurstaki

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Bacillus thuringiensis serovar kurstaki
Mg2+ activates Bacillus thuringiensis serovar kurstaki
Mn2+ highest activity in the presence of Mn2+. The addition of Mn2+ to de-metallated enzyme results in higher aldolase activity than addition of Co2+, Mg2+, or Ni2+ Bacillus thuringiensis serovar kurstaki
additional information Zn2+ does not promote activity Bacillus thuringiensis serovar kurstaki
Ni2+ activates Bacillus thuringiensis serovar kurstaki

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-deoxy-D-ribulose 1-phosphate Bacillus thuringiensis serovar kurstaki
-
 dihydroxyacetone phosphate + acetaldehyde
-
 r
5-deoxy-D-ribulose 1-phosphate Bacillus thuringiensis serovar kurstaki HD73-20
-
 dihydroxyacetone phosphate + acetaldehyde
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis serovar kurstaki P0DTQ0
-
-
Bacillus thuringiensis serovar kurstaki HD73-20 P0DTQ0
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni2+-NTA column chromatography, and Superdex 200 gel filtration Bacillus thuringiensis serovar kurstaki

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-deoxy-D-ribulose 1-phosphate
-
 Bacillus thuringiensis serovar kurstaki dihydroxyacetone phosphate + acetaldehyde
-
 r
5-deoxy-D-ribulose 1-phosphate
-
 Bacillus thuringiensis serovar kurstaki HD73-20 dihydroxyacetone phosphate + acetaldehyde
-
 r

Synonyms

Synonyms Comment Organism
5-deoxyribose disposal aldolase
-
 Bacillus thuringiensis serovar kurstaki
DrdA
-
 Bacillus thuringiensis serovar kurstaki

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3
-
 5-deoxy-D-ribulose 1-phosphate at pH 8.2 and 21°C Bacillus thuringiensis serovar kurstaki

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
 5-deoxy-D-ribulose 1-phosphate at pH 8.2 and 21°C Bacillus thuringiensis serovar kurstaki