Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.55 extracted from

  • Lamble, H.J.; Theodossis, A.; Milburn, C.C.; Taylor, G.L.;, Bull, S.D.; Hough, D.W.; Danson, M.J.
    Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus (2005), FEBS Lett., 579, 6865-6869.
    View publication on PubMed
Search for more literature for 4.1.2.55

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus
0.17
-
 2-dehydro-3-deoxy-D-galactonate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus
9.9
-
 2-dehydro-3-deoxy-D-galactonate 60°C, pH not specified in the publication Saccharolobus solfataricus
25.7
-
 2-dehydro-3-deoxy-D-gluconate 60°C, pH not specified in the publication Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus
-
 pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-galactonate 6-phosphate Saccharolobus solfataricus
-
 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus comparison of the catalytic efficiency (kcat/KM) for 2-dehydro-3-deoxy-D-gluconate (1.1 mM/s) and 2-dehydro-3-deoxy-D-gluconate 6-phosphate (643 mM/s) suggests that cleavage of the phosphorylated substrate would be significantly favoured in a situation where both compounds are present. If the 2-dehydro-3-deoxy-D-gluconate kinase is present and active then central metabolism will occur via the part-phosphorylative route.The Km of the kinase for ATP of 2.8 mM is high compared to the likely intracellular concentration, and it is possible that the non-phosphorylative pathway would be favoured under starvation conditions, which could produce pyruvate to allow the citric acid cycle to function without ATP input. regardless of whether Sulfolobus solfataricus uses the part-phosphorylative or the non-phosphorylative Entner-Doudoroff pathway, then the same enzymes can be used for the metabolism of glucose and its C4 epimer galactose pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-gluconate 6-phosphate Saccharolobus solfataricus
-
 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus O54288
-
-

Reaction

Reaction Comment Organism Reaction ID
2-dehydro-3-deoxy-6-phospho-D-galactonate = pyruvate + D-glyceraldehyde 3-phosphate (2) Saccharolobus solfataricus
a
2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate (1) Saccharolobus solfataricus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-galactonate
-
 Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-galactonate catalytic efficiency (kcat/KM) is 295fold lower compared to 2-dehydro-3-deoxy-D-galactonate 6-phosphate Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
2-dehydro-3-deoxy-D-galactonate 6-phosphate catalytic efficiency (kcat/KM) is 295fold higher compared to 2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
2-dehydro-3-deoxy-D-gluconate comparison of the catalytic efficiency (kcat/KM) for 2-dehydro-3-deoxy-D-gluconate (1.1 mM/s) and 2-dehydro-3-deoxy-D-gluconate 6-phosphate (643 mM/s) suggests that cleavage of the phosphorylated substrate would be significantly favoured in a situation where both compounds are present. If the 2-dehydro-3-deoxy-D-gluconate kinase is present and active then central metabolism will occur via the part-phosphorylative route.The Km of the kinase for ATP of 2.8 mM is high compared to the likely intracellular concentration, and it is possible that the non-phosphorylative pathway would be favoured under starvation conditions, which could produce pyruvate to allow the citric acid cycle to function without ATP input. regardless of whether Sulfolobus solfataricus uses the part-phosphorylative or the non-phosphorylative Entner-Doudoroff pathway, then the same enzymes can be used for the metabolism of glucose and its C4 epimer galactose Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-gluconate catalytic efficiency (kcat/KM) is 580fold lower compared to 2-dehydro-3-deoxy-D-gluconate 6-phosphate Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
2-dehydro-3-deoxy-D-gluconate 6-phosphate catalytic efficiency (kcat/KM) is 580fold higher compared to 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
KDG aldolase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.8
-
 2-dehydro-3-deoxy-D-galactonate 60°C, pH not specified in the publication Saccharolobus solfataricus
28.2
-
 2-dehydro-3-deoxy-D-gluconate 60°C, pH not specified in the publication Saccharolobus solfataricus
34.8
-
 2-dehydro-3-deoxy-D-galactonate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus
61.9
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function comparison of the catalytic efficiency (kcat/KM) for 2-dehydro-3-deoxy-D-gluconate (1.1 mM/s) and 2-dehydro-3-deoxy-D-gluconate 6-phosphate (643 mM/s) suggests that cleavage of the phosphorylated substrate would be significantly favoured in a situation where both compounds are present. If the 2-dehydro-3-deoxy-D-gluconate kinase is present and active then central metabolism will occur via the part-phosphorylative route.The Km of the kinase for ATP of 2.8 mM is high compared to the likely intracellular concentration, and it is possible that the non-phosphorylative pathway would be favoured under starvation conditions, which could produce pyruvate to allow the citric acid cycle to function without ATP input. regardless of whether Sulfolobus solfataricus uses the part-phosphorylative or the non-phosphorylative Entner-Doudoroff pathway, then the same enzymes can be used for the metabolism of glucose and its C4 epimer galactose Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 2-dehydro-3-deoxy-D-galactonate 60°C, pH not specified in the publication Saccharolobus solfataricus
1.1
-
 2-dehydro-3-deoxy-D-gluconate 60°C, pH not specified in the publication Saccharolobus solfataricus
207
-
 2-dehydro-3-deoxy-D-galactonate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus
643
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus