Cloned (Comment) | Organism |
---|---|
overexpressed in recombinant Escherichia coli cells | Aeromonas jandaei |
Protein Variants | Comment | Organism |
---|---|---|
K199A | catalytically inactive mutant enzyme | Aeromonas jandaei |
K224A | mutant enzyme shows properties similar to the wild type enzyme | Aeromonas jandaei |
K51A | mutant enzyme shows properties similar to the wild type enzyme | Aeromonas jandaei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
L-allo-threonine | pH 8.0, 30°C, mutant enzyme K51A | Aeromonas jandaei | |
0.37 | - |
L-allo-threonine | pH 8.0, 30°C, wild-type enzyme | Aeromonas jandaei | |
0.38 | - |
L-allo-threonine | pH 8.0, 30°C, mutant enzyme K224A | Aeromonas jandaei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36294 | - |
4 * 36294, calculated from sequence | Aeromonas jandaei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeromonas jandaei | O07051 | - |
- |
Aeromonas jandaei DK-39 | O07051 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeromonas jandaei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.4 | - |
pH 8.0, 30°C | Aeromonas jandaei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-allo-threonine | - |
Aeromonas jandaei | glycine + acetaldehyde | - |
r | |
L-allo-threonine | - |
Aeromonas jandaei DK-39 | glycine + acetaldehyde | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36294, calculated from sequence | Aeromonas jandaei |
Synonyms | Comment | Organism |
---|---|---|
L-allo-TA | - |
Aeromonas jandaei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aeromonas jandaei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
L-allo-threonine | pH 8.0, 30°C, mutant enzyme K51A | Aeromonas jandaei | |
3 | - |
L-allo-threonine | pH 8.0, 30°C, wild-type enzyme | Aeromonas jandaei | |
3 | - |
L-allo-threonine | pH 8.0, 30°C, mutant enzyme K224A | Aeromonas jandaei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Aeromonas jandaei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Lys199 probably functions as an essential catalytic residue forming an internal Schiff base with pyridoxal 5'-phosphate of the enzyme to catalyze the reversible aldol reaction | Aeromonas jandaei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.9 | - |
L-allo-threonine | pH 8.0, 30°C, mutant enzyme K224A | Aeromonas jandaei | |
8.1 | - |
L-allo-threonine | pH 8.0, 30°C, mutant enzyme K51A | Aeromonas jandaei | |
8.1 | - |
L-allo-threonine | pH 8.0, 30°C, wild-type enzyme | Aeromonas jandaei |