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Literature summary for 4.1.2.48 extracted from

  • Kumagai, H.; Nagate, T.; Yoshida, H.; Yamada, H.
    Threonine aldolase from Candida humicola. II. Purification, crystallization and properties (1972), Biochim. Biophys. Acta, 258, 779-790.
    View publication on PubMed
Search for more literature for 4.1.2.48

Activating Compound

Activating Compound Comment Organism Structure
NH4+ K+ or NH4+ required for maximal activity Vanrija humicola

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Vanrija humicola

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ inhibited activation by K+ or NH4+ Vanrija humicola
Ca2+ inhibited activation by K+ or NH4+ Vanrija humicola
Mg2+ inhibited activation by K+ or NH4+ Vanrija humicola
Na+ inhibited activation by K+ or NH4+ Vanrija humicola

Metals/Ions

Metals/Ions Comment Organism Structure
K+ K+ or NH4+ required for maximal activity Vanrija humicola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
277000
-
 ultracentrifugation Vanrija humicola

Organism

Organism UniProt Comment Textmining
Vanrija humicola
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vanrija humicola

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.91
-
 pH 8.5, 30°C Vanrija humicola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Vanrija humicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Vanrija humicola

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km: 0.00025 mM, 1 mol of pyridoxal phosphate binds 46000 g of protein Vanrija humicola