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Literature summary for 4.1.2.44 extracted from

  • Gescher, J.; Eisenreich, W.; Wörth, J.; Bacher, A.; Fuchs, G.
    Aerobic benzoyl-CoA catabolic pathway in Azoarcus evansii: studies on the non-oxygenolytic ring cleavage enzyme (2005), Mol. Microbiol., 56, 1586-1600.
    View publication on PubMed
Search for more literature for 4.1.2.44

Activating Compound

Activating Compound Comment Organism Structure
additional information addition of 1 mM thiamine diphosphate to the standard assay causes only a minimal stimulation (8%) Aromatoleum evansii

Cloned(Commentary)

Cloned (Comment) Organism
the boxC gene is expressed in a recombinant Escherichia coli strain as a fusion protein with maltose binding protein (BoxCmal) Aromatoleum evansii

Inhibitors

Inhibitors Comment Organism Structure
additional information acetoacetyl-CoA (0.2 mM), a potential inhibitor of enoyl-CoA hydratase, has no impact on enzyme activity of BoxCmal. Crotonyl-CoA (0.2 mM), a potential substrate of enoyl-CoA hydratase (crotonase), is neither converted to 3-hydroxybutyryl-CoA nor does it inhibit the standard assay Aromatoleum evansii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 2,3-dihydro-2,3-dihydroxybenzoyl-CoA
-
 Aromatoleum evansii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the purified protein does not require divalent metals or any cosubstrates or coenzymes for activity Aromatoleum evansii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Aromatoleum evansii
120000
-
 gel filtration Aromatoleum evansii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O Aromatoleum evansii the enzyme is involved in the aerobic benzoyl-CoA catabolic pathway. Benzoyl-CoA is oxidized to 2,3-dihydro-2,3-dihydroxybenzoyl-CoA (benzoyl-CoA dihydrodiol) by benzoyl-CoA oxygenase/reductase BoxBA in the presence of molecular oxygen. The next, ring cleaving step is catalysed by BoxC 3,4-dehydroadipyl-CoA semialdehyde + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Aromatoleum evansii Q84HH6
-
-

Purification (Commentary)

Purification (Comment) Organism
wild type and recombinant proteins Aromatoleum evansii

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:benzoate-grown cell
-
 Aromatoleum evansii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
-
 Aromatoleum evansii

Storage Stability

Storage Stability Organism
-20°C, the protein can be stored without appreciable loss of activity for months in the presence of 10% (v/v) glycerol Aromatoleum evansii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O the enzyme is involved in the aerobic benzoyl-CoA catabolic pathway. Benzoyl-CoA is oxidized to 2,3-dihydro-2,3-dihydroxybenzoyl-CoA (benzoyl-CoA dihydrodiol) by benzoyl-CoA oxygenase/reductase BoxBA in the presence of molecular oxygen. The next, ring cleaving step is catalysed by BoxC Aromatoleum evansii 3,4-dehydroadipyl-CoA semialdehyde + formate
-
 ?
2,3-dihydro-2,3-dihydroxybenzoyl-CoA + H2O NADPH and semicarbazide are analysed directly by NMR spectroscopy and mass spectrometry. The purified protein does not require molecular oxygen for activity Aromatoleum evansii 3,4-dehydroadipyl-CoA semialdehyde + formate
-
 ?
additional information no activity with crotonyl-CoA Aromatoleum evansii ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Aromatoleum evansii

Synonyms

Synonyms Comment Organism
benzoyl-CoA oxidation component C
-
 Aromatoleum evansii
BoxC
-
 Aromatoleum evansii
dihydrodiol transforming enzyme
-
 Aromatoleum evansii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 2,3-dihydro-2,3-dihydroxybenzoyl-CoA
-
 Aromatoleum evansii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aromatoleum evansii

pH Range

pH Minimum pH Maximum Comment Organism
7 11 half maximal activity at pH 7 and pH 11 Aromatoleum evansii

Cofactor

Cofactor Comment Organism Structure
additional information the purified protein does not require divalent metals or any cosubstrates or coenzymes for activity Aromatoleum evansii

pI Value

Organism Comment pI Value Maximum pI Value
Aromatoleum evansii
-
-
 5.6