Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
DL-threo-beta-(3,4-dihydroxyphenylserine) | pH 8.0, 30°C, with Mn2+ | Arthrobacter sp. | |
1.4 | - |
DL-threo-beta-(3,4-dihydroxyphenylserine) | pH 8.0, 30°C, without Mn2+ | Arthrobacter sp. | |
1.8 | - |
DL-threo-beta-(3,4-methylenedioxyphenylserine) | pH 8.0, 30°C, with Mn2+ | Arthrobacter sp. | |
1.8 | - |
DL-threo-beta-(3,4-methylenedioxyphenylserine) | pH 8.0, 30°C, without Mn2+ | Arthrobacter sp. | |
2 | - |
DL-erythro-beta-(3,4-methylenedioxyphenylserine) | pH 8.0, 30°C, without Mn2+ | Arthrobacter sp. | |
2.5 | - |
DL-erythro-beta-(3,4-methylenedioxyphenylserine) | pH 8.0, 30°C, with Mn2+ | Arthrobacter sp. | |
4.3 | - |
D-allo-threonine | pH 8.0, 30°C, without Mn2+ | Arthrobacter sp. | |
4.4 | - |
D-allo-threonine | pH 8.0, 30°C, with Mn2+ | Arthrobacter sp. | |
4.9 | - |
DL-erythro-phenylserine | pH 8.0, 30°C, without Mn2+ | Arthrobacter sp. | |
5.3 | - |
DL-erythro-phenylserine | pH 8.0, 30°C, with Mn2+ | Arthrobacter sp. | |
5.4 | - |
DL-threo-phenylserine | pH 8.0, 30°C, with Mn2+ | Arthrobacter sp. | |
5.9 | - |
DL-threo-phenylserine | pH 8.0, 30°C, without Mn2+ | Arthrobacter sp. | |
8.4 | - |
D-threonine | pH 8.0, 30°C, with Mn2+ | Arthrobacter sp. | |
8.8 | - |
D-threonine | pH 8.0, 30°C, without Mn2+ | Arthrobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates, can bind 1 mol of Mn2+ per mol of subunit | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | O82872 | - |
- |
Arthrobacter sp. DK-38 | O82872 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.7 | - |
- |
Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allo-threonine | - |
Arthrobacter sp. | glycine + acetaldehyde | - |
r | |
D-allo-threonine | - |
Arthrobacter sp. DK-38 | glycine + acetaldehyde | - |
r | |
D-threonine | - |
Arthrobacter sp. | glycine + acetaldehyde | - |
r | |
D-threonine | - |
Arthrobacter sp. DK-38 | glycine + acetaldehyde | - |
r | |
DL-erythro-beta-(3,4-methylenedioxyphenylserine) | - |
Arthrobacter sp. | ? | - |
r | |
DL-erythro-beta-(3,4-methylenedioxyphenylserine) | - |
Arthrobacter sp. DK-38 | ? | - |
r | |
DL-erythro-phenylserine | - |
Arthrobacter sp. | glycine + benzaldehyde | - |
r | |
DL-erythro-phenylserine | - |
Arthrobacter sp. DK-38 | glycine + benzaldehyde | - |
r | |
DL-threo-beta-(3,4-dihydroxyphenylserine) | - |
Arthrobacter sp. | ? | - |
r | |
DL-threo-beta-(3,4-dihydroxyphenylserine) | - |
Arthrobacter sp. DK-38 | ? | - |
r | |
DL-threo-beta-(3,4-methylenedioxyphenylserine) | - |
Arthrobacter sp. | ? | - |
r | |
DL-threo-phenylserine | - |
Arthrobacter sp. | glycine + benzaldehyde | - |
r | |
glycine + acetaldehyde | - |
Arthrobacter sp. | D-threonine + D-allo-threonine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
low specificity D-threonine aldolase | - |
Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | binds 1 mol of pyridoxal 5'-phosphate per mol of subunit, Lys59 is the cofactor binding site | Arthrobacter sp. |