BRENDA - Enzyme Database
show all sequences of 4.1.2.22

Purification and properties of two fructose-6-phosphate phosphoketolase in Bifidobacterium

Sgorbath, B.; Lenaz, G.; Casalicchio, F.; Antonie van Leeuwenhoek 42, 49-57 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
-
Bifidobacterium dentium
(NH4)2SO4
-
Bifidobacterium pseudolongum subsp. globosum
hydroxylamine
-
Bifidobacterium dentium
hydroxylamine
-
Bifidobacterium pseudolongum subsp. globosum
Iodosobenzoate
-
Bifidobacterium dentium
Iodosobenzoate
-
Bifidobacterium pseudolongum subsp. globosum
p-hydroxymercuribenzoate
-
Bifidobacterium dentium
p-hydroxymercuribenzoate
-
Bifidobacterium pseudolongum subsp. globosum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4
-
fructose 6-phosphate
-
Bifidobacterium pseudolongum subsp. globosum
39
-
fructose 6-phosphate
-
Bifidobacterium dentium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
45% of the activation with Mn2+
Bifidobacterium dentium
Mg2+
maximal activation in presence of Mg2+
Bifidobacterium pseudolongum subsp. globosum
Mn2+
maximal activation with Mn2+
Bifidobacterium dentium
Mn2+
44% of the activation with Mg2+
Bifidobacterium pseudolongum subsp. globosum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
160000
-
gel filtration
Bifidobacterium dentium
290000
-
gel filtration
Bifidobacterium pseudolongum subsp. globosum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bifidobacterium dentium
-
-
-
Bifidobacterium pseudolongum subsp. globosum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bifidobacterium dentium
-
Bifidobacterium pseudolongum subsp. globosum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
24
-
-
Bifidobacterium pseudolongum subsp. globosum
30
-
-
Bifidobacterium dentium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Fructose 6-phosphate + phosphate
-
5096
Bifidobacterium pseudolongum subsp. globosum
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
-
D-Fructose 6-phosphate + phosphate
-
5096
Bifidobacterium dentium
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
-
D-ribose 5-phosphate + phosphate
13.3% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium dentium
?
-
-
-
-
D-ribose 5-phosphate + phosphate
25% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
D-ribose 5-phosphate + phosphate
208% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
Fructose 1-6-diphosphate + phosphate
13.3% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium dentium
?
-
-
-
-
Fructose 1-6-diphosphate + phosphate
20.8% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
Sedoheptulose 7-phosphate + phosphate
13.3% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium dentium
?
-
-
-
-
Sedoheptulose 7-phosphate + phosphate
16.6% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
Xylulose 5-phosphate + phosphate
166% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
Xylulose 5-phosphate + phosphate
6.6% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium dentium
?
-
-
-
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
57
-
5 min, 40% loss of activity
Bifidobacterium dentium
57
-
5 min, stable
Bifidobacterium pseudolongum subsp. globosum
65
-
5 min, about 45% loss of activity
Bifidobacterium dentium
65
-
5 min, about 65% loss of activity
Bifidobacterium pseudolongum subsp. globosum
80
-
5 min, complete loss of activity
Bifidobacterium dentium
80
-
5 min, about 85% loss of activity
Bifidobacterium pseudolongum subsp. globosum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6
-
Bifidobacterium pseudolongum subsp. globosum
7
-
-
Bifidobacterium dentium
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
8
pH 4.0: about 40% of maximal activity, pH 9.0: about 30% of maximal activity
Bifidobacterium pseudolongum subsp. globosum
4
9
about 30% of maximal activity at pH 4.0 and at pH 9.0
Bifidobacterium dentium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
-
Bifidobacterium dentium
(NH4)2SO4
-
Bifidobacterium pseudolongum subsp. globosum
hydroxylamine
-
Bifidobacterium dentium
hydroxylamine
-
Bifidobacterium pseudolongum subsp. globosum
Iodosobenzoate
-
Bifidobacterium dentium
Iodosobenzoate
-
Bifidobacterium pseudolongum subsp. globosum
p-hydroxymercuribenzoate
-
Bifidobacterium dentium
p-hydroxymercuribenzoate
-
Bifidobacterium pseudolongum subsp. globosum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4
-
fructose 6-phosphate
-
Bifidobacterium pseudolongum subsp. globosum
39
-
fructose 6-phosphate
-
Bifidobacterium dentium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
45% of the activation with Mn2+
Bifidobacterium dentium
Mg2+
maximal activation in presence of Mg2+
Bifidobacterium pseudolongum subsp. globosum
Mn2+
maximal activation with Mn2+
Bifidobacterium dentium
Mn2+
44% of the activation with Mg2+
Bifidobacterium pseudolongum subsp. globosum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
160000
-
gel filtration
Bifidobacterium dentium
290000
-
gel filtration
Bifidobacterium pseudolongum subsp. globosum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bifidobacterium dentium
-
Bifidobacterium pseudolongum subsp. globosum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
24
-
-
Bifidobacterium pseudolongum subsp. globosum
30
-
-
Bifidobacterium dentium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Fructose 6-phosphate + phosphate
-
5096
Bifidobacterium pseudolongum subsp. globosum
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
-
D-Fructose 6-phosphate + phosphate
-
5096
Bifidobacterium dentium
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
-
-
-
D-ribose 5-phosphate + phosphate
13.3% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium dentium
?
-
-
-
-
D-ribose 5-phosphate + phosphate
25% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
D-ribose 5-phosphate + phosphate
208% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
Fructose 1-6-diphosphate + phosphate
13.3% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium dentium
?
-
-
-
-
Fructose 1-6-diphosphate + phosphate
20.8% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
Sedoheptulose 7-phosphate + phosphate
13.3% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium dentium
?
-
-
-
-
Sedoheptulose 7-phosphate + phosphate
16.6% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
Xylulose 5-phosphate + phosphate
166% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium pseudolongum subsp. globosum
?
-
-
-
-
Xylulose 5-phosphate + phosphate
6.6% of the activity with D-fructose 6-phosphate
5096
Bifidobacterium dentium
?
-
-
-
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
57
-
5 min, 40% loss of activity
Bifidobacterium dentium
57
-
5 min, stable
Bifidobacterium pseudolongum subsp. globosum
65
-
5 min, about 45% loss of activity
Bifidobacterium dentium
65
-
5 min, about 65% loss of activity
Bifidobacterium pseudolongum subsp. globosum
80
-
5 min, complete loss of activity
Bifidobacterium dentium
80
-
5 min, about 85% loss of activity
Bifidobacterium pseudolongum subsp. globosum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6
-
Bifidobacterium pseudolongum subsp. globosum
7
-
-
Bifidobacterium dentium
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
8
pH 4.0: about 40% of maximal activity, pH 9.0: about 30% of maximal activity
Bifidobacterium pseudolongum subsp. globosum
4
9
about 30% of maximal activity at pH 4.0 and at pH 9.0
Bifidobacterium dentium
Other publictions for EC 4.1.2.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747623
Pechar
Classification of culturable ...
Bifidobacterium boum, Bifidobacterium boum LMG 10736, Bifidobacterium boum ST6, Bifidobacterium choerinum, Bifidobacterium choerinum LMG 10510, Bifidobacterium pseudolongum subsp. globosum, Bifidobacterium pseudolongum subsp. globosum JCM 5820, Bifidobacterium pseudolongum subsp. globosum ST7, Bifidobacterium pseudolongum subsp. pseudolongum, Bifidobacterium pseudolongum subsp. pseudolongum JCM 1205, Bifidobacterium sp. ST1, Bifidobacterium sp. ST2, Bifidobacterium sp. ST3, Bifidobacterium sp. ST4, Bifidobacterium sp. ST5, Bifidobacterium thermacidophilum subsp. thermacidophilum, Bifidobacterium thermacidophilum subsp. thermacidophilum DSM 15837, Bifidobacterium thermophilum, Bifidobacterium thermophilum AS 1.2235, Bifidobacterium thermophilum subsp. porcinum, Bifidobacterium thermophilum subsp. porcinum P3-14, Bifidobacterium thermophilum subsp. porcinum ST8, Bifidobacterium thermophilum subsp. porcinum ST9
Curr. Microbiol.
74
1324-1331
2017
-
-
-
-
-
-
-
-
-
-
-
23
-
28
-
-
-
-
-
-
-
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23
-
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-
-
-
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23
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-
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747991
Pechar
Bifidobacterium apri sp. nov. ...
Bifidobacterium apri, Bifidobacterium apri DSM 100238
Int. J. Syst. Evol. Microbiol.
67
2349-2356
2017
-
-
-
-
-
-
-
-
-
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-
2
-
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-
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-
-
-
-
-
-
-
747992
Sechovcova
Alloscardovia venturai sp. no ...
Alloscardovia venturai, Alloscardovia venturai DSM 100237
Int. J. Syst. Evol. Microbiol.
67
2842-2847
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749102
Gupta
Novel molecular, structural a ...
Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium reuteri
PLoS ONE
12
e0172176
2017
-
-
-
-
-
-
-
-
-
-
-
10
-
5
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
10
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746713
Bergman
Functional expression and eva ...
Bifidobacterium adolescentis, Bifidobacterium animalis subsp. lactis, Bifidobacterium breve, Clostridium acetobutylicum, Lactobacillus plantarum, Leuconostoc mesenteroides
AMB Express
6
115
2016
-
6
6
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
7
7
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
6
7
-
-
-
747315
Moriyama
Characterization of three put ...
Nostoc sp. PCC 7120
Biosci. Biotechnol. Biochem.
79
767-774
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
1
-
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2
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2
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1
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748094
Glenn
Allosteric regulation of Lact ...
Lactobacillus plantarum
J. Bacteriol.
197
1157-1163
2015
-
-
1
-
-
-
4
1
-
-
-
2
-
4
-
-
1
-
-
-
-
-
2
-
-
-
-
2
1
-
-
1
-
-
3
-
-
1
1
-
-
-
3
4
-
1
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
2
1
-
-
-
-
-
-
-
2
2
748512
Taylor
Biolistic transformation of a ...
Cryptococcus neoformans
J. Vis. Exp.
97
e52666
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
3
-
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1
-
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-
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1
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3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748753
Xiong
Phosphoketolase pathway contr ...
Synechocystis sp. DELTAglgC/xylAB
Nat. Plants
2
15187
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727394
Glenn
Biochemical and kinetic charac ...
Cryptococcus neoformans
Eukaryot. Cell
13
657-663
2014
1
-
-
-
-
-
3
-
-
-
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4
-
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1
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1
1
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-
1
3
-
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-
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-
-
-
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-
-
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1
-
-
-
-
-
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-
-
-
746881
Petrareanu
Phosphoketolases from Lactoco ...
Lactococcus lactis, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis IL1403, Leuconostoc mesenteroides, Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc mesenteroides subsp. mesenteroides J18, Pseudomonas aeruginosa
Appl. Microbiol. Biotechnol.
98
7855-7867
2014
-
-
5
-
-
-
-
10
-
-
-
6
-
8
-
-
5
-
-
-
-
-
16
3
3
3
-
12
3
-
-
5
-
1
-
-
-
6
6
-
-
-
-
-
-
12
-
-
-
6
-
-
-
6
-
-
-
-
16
3
3
3
-
12
3
-
-
1
-
-
-
-
10
12
747246
Tittmann
Sweet siblings with different ...
Bifidobacterium breve
Bioorg. Chem.
57
263-280
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
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-
-
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2
-
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1
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1
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2
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728652
Bolado-Martinez
Fructose 6-phosphate phosphoke ...
Lactobacillus sp.
Prikl. Biokhim. Mikrobiol.
48
494-500
2012
-
-
-
-
-
-
-
-
-
-
-
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