BRENDA - Enzyme Database show
show all sequences of 4.1.2.20

2-keto-3-deoxy-D-Glucarate aldolase

Fish, D.C.; Blumenthal, H.J.; Methods Enzymol. 9, 529-534 (1966)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CN-
reversed by Mg2+ and Co2+, but not by Fe2+
Escherichia coli
diphosphate
-
Escherichia coli
EDTA
-
Escherichia coli
F-
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Fe2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Mg2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Mn2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Mo2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli CR63MA
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-D-glucarate
-
5078
Escherichia coli
pyruvate + tartronate semialdehyde
-
5078
Escherichia coli
-
2-dehydro-3-deoxy-D-glucarate
-
5078
Escherichia coli CR63MA
pyruvate + tartronate semialdehyde
-
5078
Escherichia coli CR63MA
-
5-Dehydro-4-deoxy-D-glucarate
-
5078
Escherichia coli
Pyruvate + tartronate semialdehyde
-
5078
Escherichia coli
-
5-Dehydro-4-deoxy-D-glucarate
-
5078
Escherichia coli CR63MA
Pyruvate + tartronate semialdehyde
-
5078
Escherichia coli CR63MA
-
pyruvate + glycolaldehyde
-
5078
Escherichia coli
2-oxo-4,5-dihydroxy-L-pentanoic acid
-
5078
Escherichia coli
-
pyruvate + glycolaldehyde
-
5078
Escherichia coli CR63MA
2-oxo-4,5-dihydroxy-L-pentanoic acid
-
5078
Escherichia coli CR63MA
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
8.6
-
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.5
stable
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CN-
reversed by Mg2+ and Co2+, but not by Fe2+
Escherichia coli
diphosphate
-
Escherichia coli
EDTA
-
Escherichia coli
F-
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Fe2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Mg2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Mn2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Mo2+
absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydro-3-deoxy-D-glucarate
-
5078
Escherichia coli
pyruvate + tartronate semialdehyde
-
5078
Escherichia coli
-
2-dehydro-3-deoxy-D-glucarate
-
5078
Escherichia coli CR63MA
pyruvate + tartronate semialdehyde
-
5078
Escherichia coli CR63MA
-
5-Dehydro-4-deoxy-D-glucarate
-
5078
Escherichia coli
Pyruvate + tartronate semialdehyde
-
5078
Escherichia coli
-
5-Dehydro-4-deoxy-D-glucarate
-
5078
Escherichia coli CR63MA
Pyruvate + tartronate semialdehyde
-
5078
Escherichia coli CR63MA
-
pyruvate + glycolaldehyde
-
5078
Escherichia coli
2-oxo-4,5-dihydroxy-L-pentanoic acid
-
5078
Escherichia coli
-
pyruvate + glycolaldehyde
-
5078
Escherichia coli CR63MA
2-oxo-4,5-dihydroxy-L-pentanoic acid
-
5078
Escherichia coli CR63MA
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
8.6
-
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.5
stable
Escherichia coli
Other publictions for EC 4.1.2.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716878
Bhaskar
Identification of biochemical ...
Escherichia coli
Proteins
79
1132-1142
2011
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1
1
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4
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1
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2
1
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1
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1
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1
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2
1
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1
1
-
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-
704334
Reher
The nonphosphorylative Entner- ...
Picrophilus torridus
J. Bacteriol.
192
964-974
2010
-
-
1
-
-
-
-
2
-
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2
-
-
3
-
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1
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1
2
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2
1
1
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1
2
1
1
-
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1
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-
2
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2
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1
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1
2
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2
1
1
-
1
2
1
1
-
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1
1
-
2
2
691007
Rea
Crystal structure and function ...
Escherichia coli
Biochemistry
47
9955-9965
2008
-
-
1
1
-
-
-
7
-
2
-
-
-
2
-
-
1
-
-
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6
-
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7
1
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-
1
-
1
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7
-
2
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1
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-
6
-
-
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7
1
-
-
-
-
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-
677338
Li
Purification, crystallization ...
Leptospira interrogans
Acta Crystallogr. Sect. F
62
1269-1270
2006
-
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1
1
-
-
-
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-
2
-
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2
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1
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1
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1
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1
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1
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1
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650974
Izard
Crystal structures of the meta ...
Escherichia coli
EMBO J.
19
3849-3856
2000
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-
1
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-
3
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2
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1
1
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1
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1
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3
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1
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1
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649100
Blackwell
-
Rhombohedral crystals of 2-deh ...
Escherichia coli
Acta Crystallogr. Sect. D
55
1368-1369
1999
-
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-
1
-
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1
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1
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1
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1
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1
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1
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1
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1
-
1
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1
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5083
Hubbard
Evolution of enzymatic activit ...
Escherichia coli
Biochemistry
37
14369-14375
1998
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1
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1
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2
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2
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1
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2
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1
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1
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1
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1
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2
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1
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5082
Blumenthal
Specific enzymatic assay for D ...
Escherichia coli
Anal. Biochem.
185
286-293
1990
-
1
-
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-
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1
-
1
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3
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1
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1
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3
-
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-
5081
Marsh
An enzymatic determination of ...
Escherichia coli, Escherichia coli NCTC 10418
Anal. Biochem.
145
266-272
1985
-
1
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2
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2
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1
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2
-
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5080
Jeffcoat
Bacterial hydrolases and aldol ...
Klebsiella aerogenes
Nature New Biol.
241
186-187
1973
-
-
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1
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1
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1
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1
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1
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4971
Wood
-
2-Keto-3-deoxy-6-phosphoglucon ...
Escherichia coli, no activity in Bacillus subtilis
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
281-302
1972
-
-
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5
-
1
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2
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8
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5
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1
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8
-
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-
-
-
-
-
-
-
-
-
-
-
5078
Fish
-
2-keto-3-deoxy-D-Glucarate ald ...
Escherichia coli, Escherichia coli CR63MA
Methods Enzymol.
9
529-534
1966
-
-
-
-
-
-
4
1
-
5
-
-
-
4
-
-
1
-
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-
1
-
6
-
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-
1
-
1
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4
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1
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5
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1
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1
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6
-
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1
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1
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