Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | up to 5 mM, enhances enzyme activity twofold | Haloferax mediterranei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 2 mM, 85% inhibition | Haloferax mediterranei | |
2,2'-bipyridyl | 2 mM, 76% inhibition | Haloferax mediterranei | |
EDTA | 2 mM, complete inhibition. Activity can be completely restored only by Fe2+. Other divalent metal ions such as Mn2+, Mg2+, and Zn2+ have no effect. Co2+ and Ca2+ can restore 20% and 10%, respectively, of enzyme activity | Haloferax mediterranei | |
Fe2+ | optimum aldolase activity (137%) is observed with 1.0 mM Fe2+ concentration. Inhibition at concentrations baove 1.0 mM | Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 37°C | Haloferax mediterranei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can restore 10% of enzyme activity after complete inhibition by EDTA treatment (2 mM) | Haloferax mediterranei | |
Co2+ | can restore 20% of enzyme activity after complete inhibition by EDTA treatment (2 mM) | Haloferax mediterranei | |
Fe2+ | optimum aldolase activity (137%) is observed with 1.0 mM Fe2+ concentration. Inhibition at concentrations above 1.0 mM. Activity of the enzyme completely inhibited by EDTA (2 mM) can be completely restored by Fe2+ | Haloferax mediterranei | |
KCl | aldolase activity increases with an increasing concentration of KCl. At 4.0 M KCl concentration, activity is 2.5 times that at 0.5 M KCl concentration. Enzyme activity is appreciable even in the absence of added salt | Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Haloferax mediterranei |
110000 | - |
sucrose density gradient ultracentrifugation | Haloferax mediterranei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Haloferax mediterranei | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | Haloferax mediterranei DSM 1411 | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | - |
- |
- |
Haloferax mediterranei DSM 1411 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
pH 7.5, 37°C | Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Haloferax mediterranei | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | - |
Haloferax mediterranei DSM 1411 | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE | Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
class II fructose-1,6-bisphosphate aldolase | - |
Haloferax mediterranei |
FBP aldolase | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | broad pH optimum in glycylglycine buffer and imidazole buffer | Haloferax mediterranei |
7.5 | - |
in Tris-HCl buffer | Haloferax mediterranei |