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Literature summary for 4.1.2.10 extracted from

  • Lanfranchi, E.; Pavkov-Keller, T.; Koehler, E.M.; Diepold, M.; Steiner, K.; Darnhofer, B.; Hartler, J.; Van Den Bergh, T.; Joosten, H.J.; Gruber-Khadjawi, M.; Thallinger, G.G.; Birner-Gruenberger, R.; Gruber, K.; Winkler, M.; Glieder, A.
    Enzyme discovery beyond homology a unique hydroxynitrile lyase in the Bet v1 superfamily (2017), Sci. Rep., 7, 46738 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.10

Cloned(Commentary)

Cloned (Comment) Organism
-
 Davallia tyermanii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor-diffusion method Davallia tyermanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL1 Davallia tyermanii
0.45
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL2 Davallia tyermanii
0.63
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL4 Davallia tyermanii
0.75
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL3 Davallia tyermanii
14
-
 benzaldehyde pH 4.0, 10°C, enzyme form DtHNL1 Davallia tyermanii

Organism

Organism UniProt Comment Textmining
Davallia tyermanii A0A1C9V3R0 hydroxynitrile lyase isoform 2
-
Davallia tyermanii A0A1C9V3R4 hydroxynitrile lyase isoform 3
-
Davallia tyermanii A0A1C9V3R6 hydroxynitrile lyase isoform 4
-
Davallia tyermanii A0A1C9V3S9 hydroxynitrile lyase isoform 1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Davallia tyermanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelonitrile
-
 Davallia tyermanii cyanide + benzaldehyde
-
 r
cyanide + 1-phenylethanone
-
 Davallia tyermanii 2-hydroxy-2-phenylpropanenitrile
-
 ?
cyanide + 2-chlorobenzaldehyde
-
 Davallia tyermanii (2R)-2-(2-chlorophenyl)-2-hydroxyacetonitrile
-
 ?
cyanide + 3-phenylprop-2-enal
-
 Davallia tyermanii (2R)-2-hydroxy-4-phenylbut-3-enenitrile
-
 ?
cyanide + 3-phenylpropanal
-
 Davallia tyermanii (2R)-2-hydroxy-4-phenylbutanenitrile
-
 ?
cyanide + benzaldehyde
-
 Davallia tyermanii (R)-mandelonitrile
-
 r
cyanide + furan-2-carbaldehyde
-
 Davallia tyermanii (2S)-furan-2-yl-hydroxyacetonitrile
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 enzyme form DtHNL2 Davallia tyermanii
35
-
 enzyme form DtHNL4 Davallia tyermanii
40
-
 enzyme form DtHNL1 Davallia tyermanii
40
-
 enzyme form DtHNL3 Davallia tyermanii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
144
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL1 Davallia tyermanii
156
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL2 Davallia tyermanii
272
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL4 Davallia tyermanii
356
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL3 Davallia tyermanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Davallia tyermanii

pH Range

pH Minimum pH Maximum Comment Organism
2.5 4 active at pH 2.5, about 80% of maximal activity at pH 4.0 Davallia tyermanii
2.5 4 active at pH 2.5, about 80% of maximal activity at pH 4.0< Davallia tyermanii

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5
-
 8°C, 72 h, more than 50% of activity is retained Davallia tyermanii
2.5
-
 8°C, 72 h, more than 50% of activity retained Davallia tyermanii
2.5
-
 8°C, 72 h, more than 50% of activity retains Davallia tyermanii
4
-
 8°C, 72 h, more than 50% of activity is retained Davallia tyermanii
4
-
 8°C, 72 h, more than 50% of activity retained Davallia tyermanii
4
-
 8°C, 72 h, more than 50% of activity retains Davallia tyermanii
5
-
 8°C, 72 h, more than 65% of activity is retained Davallia tyermanii
5
-
 8°C, 72 h, more than 65% of activity retained Davallia tyermanii
5
-
 8°C, 72 h, more than 65% of activity retains Davallia tyermanii

General Information

General Information Comment Organism
metabolism the enzyme is involved in the cyanogenic pathway, a widespread defense mechanism against herbivores and pathogens. Plants produce cyanogenic glycosides e.g. prunasin or amygdalin, store these compounds in different tissues, and enzymatically degrade them upon tissue disruption, in order to finally release toxic hydrogen cyanide Davallia tyermanii
physiological function the enzyme is involved in the cyanogenic pathway, a widespread defense mechanism against herbivores and pathogens. Plants produce cyanogenic glycosides e.g. prunasin or amygdalin, store these compounds in different tissues, and enzymatically degrade them upon tissue disruption, in order to finally release toxic hydrogen cyanide Davallia tyermanii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5
-
 (R)-mandelonitrile pH 5.0, temperature not specified in the publication, enzyme form DtHNL1 Davallia tyermanii