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Literature summary for 4.1.2.10 extracted from

  • Yao, L.; Li, H.; Yang, J.; Li, C.; Shen, Y.
    Purification and characterization of a hydroxynitrile lyase from Amygdalus pedunculata Pall (2018), Int. J. Biol. Macromol., 118, 189-194 .
    View publication on PubMed
Search for more literature for 4.1.2.10

Application

Application Comment Organism
synthesis Amygdalus pedunculata hydroxynitrile lyase is an excellent biocatalyst and has very high potential for synthesis of enantiopure cyanohydrins Prunus pedunculata

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 50 mM, 43% loss of activity Prunus pedunculata
Al3+ 50 mM, 26% loss of activity Prunus pedunculata
Ba2+ 50 mM, 6% loss of activity Prunus pedunculata
Cd2+ 50 mM, 27% loss of activity Prunus pedunculata
Cu2+ 50 mM, 23% loss of activity Prunus pedunculata
Fe2+ 50 mM, 6% loss of activity Prunus pedunculata
Fe3+ 50 mM, 4% loss of activity Prunus pedunculata
Hg2+ 50 mM, 54% loss of activity Prunus pedunculata
K+ 50 mM, 11% loss of activity Prunus pedunculata
Mg2+ 50 mM, 32% loss of activity Prunus pedunculata
Mn4+ 50 mM, 39% loss of activity Prunus pedunculata
Pb2+ 50 mM, 16% loss of activity Prunus pedunculata
Sn2+ 50 mM, 14% loss of activity Prunus pedunculata
Sr2+ 50 mM, 13% loss of activity Prunus pedunculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 (R)-mandelonitrile pH 5.5, 50°C Prunus pedunculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 MALDI-TOF mass spectrometry Prunus pedunculata

Organism

Organism UniProt Comment Textmining
Prunus pedunculata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Prunus pedunculata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
661
-
 pH 5.5, 50°C Prunus pedunculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelonitrile
-
 Prunus pedunculata cyanide + benzaldehyde
-
 r
cyanide + 3-phenoxybenzaldehyde synthesis of (R)-2-hydroxy-2-(3-phenoxyphenyl)-acetonitrile with 93% enantiomeric excess Prunus pedunculata (R)-2-hydroxy-2-(3-phenoxy-phenyl)-acetonitrile
-
 ?
cyanide + benzaldehyde synthesis of (R)-mandelonitrile with 94% enantiomeric excess Prunus pedunculata (R)-mandelonitrile
-
 r

Subunits

Subunits Comment Organism
monomer
-
 Prunus pedunculata

Synonyms

Synonyms Comment Organism
APHNL
-
 Prunus pedunculata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Prunus pedunculata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70 10°C: about 70% of maximal activity, 70°C: about 60% of maximal activity Prunus pedunculata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, stable up to Prunus pedunculata
70
-
 1 h, 30% loss of activity Prunus pedunculata
80
-
 1 h, 70% loss of activity Prunus pedunculata
90
-
 1 h, 85% loss of activity Prunus pedunculata
100
-
 1 h, complete loss of activity Prunus pedunculata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
676.5
-
 (R)-mandelonitrile pH 5.5, 50°C Prunus pedunculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Prunus pedunculata

pH Range

pH Minimum pH Maximum Comment Organism
4 8 4.0 pH: about 60% of maximal activity, pH 8.0: about 50% of maximal activity Prunus pedunculata

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 1 h, complete loss of activity Prunus pedunculata
5
-
 1 h, 25% loss of activity Prunus pedunculata
6 12 1 h, enzyme is stable Prunus pedunculata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1353
-
 (R)-mandelonitrile pH 5.5, 50°C Prunus pedunculata