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Literature summary for 4.1.2.10 extracted from
- Hydroxynitrile lyase of wild apricot (Prunus armeniaca L.) purification, characterization and application in synthesis of enantiopure mandelonitrile (2017), Catal. Lett., 146, 1118-1127 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | the enzyme has very high potential for synthesis of cyanohydrins and can be used for the production of enantiopure cyanohydrins. Cyanohydrins are important intermediates in the production of pharmaceuticals and agrochemicals | Prunus armeniaca |
| pharmacology | the enzyme has very high potential for synthesis of cyanohydrins and can be used for the production of enantiopure cyanohydrins. Cyanohydrins are important intermediates in the production of pharmaceuticals and agrochemicals | Prunus armeniaca |
| synthesis | the enzyme has very high potential for synthesis of cyanohydrins and can be used for the production of enantiopure cyanohydrins. Cyanohydrins are important intermediates in the production of pharmaceuticals and agrochemicals | Prunus armeniaca |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 1% inhibition | Prunus armeniaca |  |
| Cd2+ | 10 mM, 3% inhibition | Prunus armeniaca | |
| Co2+ | 10 mM, 22% inhibition | Prunus armeniaca | |
| Cs2+ | 10 mM, 11% inhibition | Prunus armeniaca | |
| Cu2+ | 10 mM, 21% inhibition | Prunus armeniaca | |
| Fe2+ | 10 mM, 29% inhibition | Prunus armeniaca | |
| Fe3+ | 10 mM, 22% inhibition | Prunus armeniaca | |
| Hg2+ | 10 mM, complete inhibition; 10 mM, % inhibition | Prunus armeniaca | |
| Mg2+ | 10 mM, 10% inhibition | Prunus armeniaca | |
| Mn2+ | 10 mM, 17% inhibition | Prunus armeniaca | |
| Pb2+ | 10 mM, 37% inhibition | Prunus armeniaca | |
| Sr2+ | 10 mM, 15% inhibition | Prunus armeniaca | |
| W6+ | 10 mM, 5% inhibition | Prunus armeniaca | |
| Zn2+ | 10 mM, 2% inhibition | Prunus armeniaca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.76 | - |
(R)-mandelonitrile | pH 5.0, 25°C | Prunus armeniaca | |
| 16.1 | - |
benzaldehyde | pH 5.0, 25°C | Prunus armeniaca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 360000 | - |
gel filtration, native-PAGE | Prunus armeniaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prunus armeniaca | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Prunus armeniaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kernel | - |
Prunus armeniaca | - |
| seed | - |
Prunus armeniaca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 141 | - |
pH 5.0, 25°C | Prunus armeniaca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-mandelonitrile | - |
Prunus armeniaca | cyanide + benzaldehyde | - |
r | |
| cyanide + benzaldehyde | synthesis of mandelonitrile is carried out with 89% molar conversion with 96% enantionmeric excess for R-mandelonitrile | Prunus armeniaca | (R)-mandelonitrile | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000 + x * 37000, SDS-PAGE | Prunus armeniaca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ParsHNL | - |
Prunus armeniaca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Prunus armeniaca |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 55% of maximal activity, 50°C: about 25% of maximal activity | Prunus armeniaca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
the enzyme does not show any significant loss of activity at 4°C, hence it has good potential for industrial application | Prunus armeniaca |
| 25 | - |
t1/2: 216 h | Prunus armeniaca |
| 30 | - |
t1/2: 141 h | Prunus armeniaca |
| 50 | - |
t1/2: 8.1 h | Prunus armeniaca |
| 60 | - |
t1/2: 2.5 h | Prunus armeniaca |
| 70 | - |
t1/2: 0.77 h | Prunus armeniaca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 43.3 | - |
benzaldehyde | pH 5.0, 25°C | Prunus armeniaca | |
| 1130.4 | - |
(R)-mandelonitrile | pH 5.0, 25°C | Prunus armeniaca | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.75 | 5 | the enzyme shows maximum activity in 0.1 M sodium-citrate buffer having pH 4.75 at 25°C | Prunus armeniaca |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | FAD containing enzyme | Prunus armeniaca | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
benzaldehyde | pH 5.0, 25°C | Prunus armeniaca | |
| 300.6 | - |
(R)-mandelonitrile | pH 5.0, 25°C | Prunus armeniaca | |
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