Literature summary for 4.1.2.10 extracted from

Yildirim, D.; Tuekel, S.S.; Alagoez, D.
Crosslinked enzyme aggregates of hydroxynitrile lyase partially purified from Prunus dulcis seeds and its application for the synthesis of enantiopure cyanohydrins (2014), Biotechnol. Prog., 30, 818-827.

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Application

Application	Comment	Organism
synthesis	purification of enzyme as crosslinked enzyme aggregates and application for synthesis of enantiopure cyanohydrins	Prunus dulcis

Organism

Organism	UniProt	Comment	Textmining
Prunus dulcis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partly, as crosslinked enzyme aggregates. Optimal conditions are initial glutaraldehyde concentration of 25% w/v, ammonium sulfate saturation concentration of 43% w/v, and crosslinking time of 18 h	Prunus dulcis

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Prunus dulcis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-chlorobenzaldehyde + HCN	-	Prunus dulcis	(R)-2-chloromandelonitrile	after 96 h, 100% yield, 21% enantiomeric excess	?
3,4-dihydroxybenzaldehyde + HCN	-	Prunus dulcis	(R)-3,4-dihydroxymandelonitrile	after 96 h, 100% yield, 99% enantiomeric excess	?
3-phenylpropionaldehyde + HCN	-	Prunus dulcis	(R)-2-hydroxy-4-phenylbutyronitrile	after 96 h, 83% yield, 91% enantiomeric excess	?
4-bromobenzaldehyde + HCN	-	Prunus dulcis	(R)-4-bromomandelonitrile	after 96 h, 100% yield, 99% enantiomeric excess	?
4-fluorobenzaldehyde + HCN	-	Prunus dulcis	(R)-4-fluoromandelonitrile	after 96 h, 100% yield, 72% enantiomeric excess	?
4-nitrobenzaldehyde + HCN	-	Prunus dulcis	(R)-4-nitromandelonitrile	after 96 h, 100% yield, 14% enantiomeric excess	?
benzaldehyde + HCN	-	Prunus dulcis	(R)-mandelonitrile	after 96 h, 100% yield, 99% enantiomeric excess	?

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Release 2023.1 (January 2023)