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Literature summary for 4.1.2.10 extracted from

  • Tuekel, S.; Yildirim, D.; Alagoez, D.; Alptekin, O.; Yuecebilgi, G.; Bilgin, R.
    Partial purification and immobilization of a new (R)-hydroxynitrile lyase from seeds of Prunus pseudoarmeniaca (2010), J. Mol. Catal. B, 66, 161-165.
No PubMed abstract available
Search for more literature for 4.1.2.10

General Stability

General Stability Organism
both of immobilized enzymes (Eupergit C and Eupergit C250L) are used repeatedly 20times and the residual activities are about 97% of their initial activities Prunus pseudoarmeniaca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.03
-
 (R)-mandelonitrile enzyme immobilized onto Eupergit C 250L, in 50 mM citrate buffer, pH 6.0, 25°C Prunus pseudoarmeniaca
1.6
-
 (R)-mandelonitrile enzyme immobilized onto Eupergit C, in 50 mM citrate buffer, pH 6.0, 25°C Prunus pseudoarmeniaca
2.23
-
 (R)-mandelonitrile free enzyme, in 50 mM citrate buffer, pH 6.0, 25°C Prunus pseudoarmeniaca

Organism

Organism UniProt Comment Textmining
Prunus pseudoarmeniaca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation Prunus pseudoarmeniaca

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Prunus pseudoarmeniaca
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelonitrile
-
 Prunus pseudoarmeniaca cyanide + benzaldehyde
-
 r
cyanide + benzaldehyde
-
 Prunus pseudoarmeniaca (R)-mandelonitrile
-
 r

Synonyms

Synonyms Comment Organism
(R)-hydroxynitrile lyase
-
 Prunus pseudoarmeniaca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 free and immobilized enzyme Prunus pseudoarmeniaca

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 free enzyme retains about 57% of its maximum activity at 50°C whereas immobilized enzymes onto Eupergit C and Eupergit C 250 L retain about 75 and 82% of their maximum activities, respectively, at 50°C Prunus pseudoarmeniaca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 20 free and immobilized enzyme preparations show optimal carboligation activity at 5°C. As the temperature is increased from 5 to 20°C, the yields decrease, however enantiomeric excess values (99%) unchange Prunus pseudoarmeniaca
25 50 the half lives of free enzyme at 25 and 50°C are 49.9 and 30.5 h, respectively and these correspondingly are 96.3 and 43.6 h for immobilized enzyme onto Eupergit C, and 138.6 and 50.2 h for immobilized enzyme onto Eupergit C 250 L Prunus pseudoarmeniaca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 optimum pH for free and immobilized enzyme Prunus pseudoarmeniaca

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 increasing the pH value above 4.0, the yield and ee are dramatically decreased due to the spontaneous chemical decomposition of the product Prunus pseudoarmeniaca