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Literature summary for 4.1.2.10 extracted from
- Andexer, J.N.; Sehl, T.; von Langermann, J.; Frindi-Wosch, I.; Rosenkranz, T.; Fitter, J.; Gruber, K.; Kragl, U.; Eggert, T.; Pohl, M.: Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold (2009), J. Biotechnol., 141, 166-173.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is stabilized by addition of sorbitol and saccharose | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by acetate | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
(R)-4-hydroxymandelonitrile | pH 5.0, 25°C | Arabidopsis thaliana |  |
| 6 | - |
benzaldehyde | pH 6.0 | Arabidopsis thaliana | |
| 6 | - |
4-hydroxybenzaldehyde | pH 5.0, 25°C | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-4-hydroxymandelonitrile | - |
Arabidopsis thaliana | cyanide + 4-hydroxybenzaldehyde | - |
r | |
| (R)-mandelonitrile | - |
Arabidopsis thaliana | cyanide + benzaldehyde | - |
r | |
| cyanide + 4-hydroxybenzaldehyde | - |
Arabidopsis thaliana | (R)-4-hydroxymandelonitrile | - |
r | |
| additional information | low activity towards acetone cyanohydrin | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | the enzyme forms dimers in solution | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtHNL | - |
Arabidopsis thaliana |
| R-hydroxynitrile lyase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
20 | stable | Arabidopsis thaliana |
| 10 | - |
half-life time: more than 96 h | Arabidopsis thaliana |
| 20 | - |
half-life time: 80 h | Arabidopsis thaliana |
| 30 | - |
half-life time: 33 h | Arabidopsis thaliana |
| 37 | - |
half-life time: 6.6 h | Arabidopsis thaliana |
| 37 | - |
half-life 6.6 h | Arabidopsis thaliana |
| 50 | - |
half-life time: 0.3 h | Arabidopsis thaliana |
| 50 | - |
half-life 0.3 h | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 6.5 | - |
Arabidopsis thaliana |
| 5.8 | 6.5 | - |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
almost inactive below | Arabidopsis thaliana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
half-life time: 2 h, the deactivation of the enzyme at slightly acidic pH is a result of pronounced structural unfolding | Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | isoelectric focusing | - |
5.3 |
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