Literature summary for 4.1.2.10 extracted from

Pscheidt, B.; Avi, M.; Gaisberger, R.; Hartner, F.S.; Skranc, W.; Glieder, A.
Screening hydroxynitrile lyases for (R)-pantolactone synthesis (2008), J. Mol. Catal. B, 52-53, 183-188.

No PubMed abstract available

Search for more literature for 4.1.2.10

Application

Application	Comment	Organism
synthesis	production of (R)-hydroxypivalaldehyde cyanohydrin, the chiral key precursor in hydroxynitrile lyase based (R)-pantlactone synthesis	Prunus dulcis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris	Prunus dulcis

Organism

Organism	UniProt	Comment	Textmining
Prunus dulcis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyanide + 3-hydroxy-2,2-dimethylpropanal	i.e. hydroxypivaldehyde	Prunus dulcis	(2R)-2,5-dihydroxy-3,3-dimethylpentanenitrile	best enantiomeric excess is obtained at pH 2.5	?

Synonyms

Synonyms	Comment	Organism
(R)-HNL	-	Prunus dulcis
(R)-hydroxynitrile lyase	-	Prunus dulcis
Hydroxynitrile lyase	-	Prunus dulcis
PaHNL5	recombinant	Prunus dulcis
R-selective hydroxynitrile lyase	-	Prunus dulcis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.5	-	at low pH very low amounts of crude enzyme catalyse stereoselective hydroxypivalaldehyde cyanohydrin formation in water based reaction systems	Prunus dulcis

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Release 2023.1 (January 2023)