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Literature summary for 4.1.2.10 extracted from

  • Weis, R.; Poechlauer, P.; Bona, R.; Skranc, W.; Luiten, R.; Wubbolts, M.; Schwab, H.; Glieder, A.
    Biocatalytic conversion of unnatural substrates by recombinant almond R-HNL isoenzyme 5 (2004), J. Mol. Catal. B, 29, 211-218.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Pichia pastoris Prunus dulcis

Organism

Organism UniProt Comment Textmining
Prunus dulcis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Prunus dulcis

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Prunus dulcis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanide + 2-chlorobenzaldehyde
-
Prunus dulcis (2R)-(2-chlorophenyl)(hydroxy)ethanenitrile 15.8% yield and 66% enantiomeric excess after 2 h ?
cyanide + benzaldehyde
-
Prunus dulcis (R)-mandelonitrile 90.3% yield and 100% enantiomeric excess after 2 h ?

Synonyms

Synonyms Comment Organism
(R)-HNL5
-
Prunus dulcis
PaHNL5
-
Prunus dulcis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Prunus dulcis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Prunus dulcis