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Literature summary for 4.1.1.99 extracted from

  • Vannice, J.C.; Skaff, D.A.; Keightley, A.; Addo, J.K.; Wyckoff, G.J.; Miziorko, H.M.
    Identification in Haloferax volcanii of phosphomevalonate decarboxylase and isopentenyl phosphate kinase as catalysts of the terminal enzyme reactions in an archaeal alternate mevalonate pathway (2014), J. Bacteriol., 196, 1055-1063.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Haloferax volcanii H1209 Haloferax volcanii

Inhibitors

Inhibitors Comment Organism Structure
6-fluoromevalonate 5'-phosphate negligible inhibition by 6-fluoromevalonate diphosphate (a potent inhibitor of the classical mevalonate pathway), reinforcing its selectivity for monophosphorylated ligands Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 mevalonate 5-phosphate pH 7.5, 30°C Haloferax volcanii
0.289
-
 ATP pH 7.5, 30°C Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activity is not strongly dependent on KCl over a 0 to 4 M range Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37328
-
 x * 37328, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + mevalonate 5-phosphate Haloferax volcanii the enzyme is required for the alternate mevalonate pathway in an archaea ADP + phosphate + isopentenyl phosphate + CO2
-
 ?
ATP + mevalonate 5-phosphate Haloferax volcanii DSM 3757 the enzyme is required for the alternate mevalonate pathway in an archaea ADP + phosphate + isopentenyl phosphate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GXZ3
-
-
Haloferax volcanii DSM 3757 D4GXZ3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + mevalonate 5-phosphate the enzyme is required for the alternate mevalonate pathway in an archaea Haloferax volcanii ADP + phosphate + isopentenyl phosphate + CO2
-
 ?
ATP + mevalonate 5-phosphate very weak activity with mevalonate and even less with mevalonate 5-diphosphate Haloferax volcanii ADP + phosphate + isopentenyl phosphate + CO2
-
 ?
ATP + mevalonate 5-phosphate the enzyme is required for the alternate mevalonate pathway in an archaea Haloferax volcanii DSM 3757 ADP + phosphate + isopentenyl phosphate + CO2
-
 ?
ATP + mevalonate 5-phosphate very weak activity with mevalonate and even less with mevalonate 5-diphosphate Haloferax volcanii DSM 3757 ADP + phosphate + isopentenyl phosphate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 37328, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii

Synonyms

Synonyms Comment Organism
HVO_1412
-
 Haloferax volcanii
PMD
-
 Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Haloferax volcanii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.016
-
 pH 7.5, 30°C Haloferax volcanii 6-fluoromevalonate 5'-phosphate