Application | Comment | Organism |
---|---|---|
synthesis | selective and ecological production by carboxylation of phenol to form salicylate, the enzymatic KolbeSchmitt reaction | Cutaneotrichosporon moniliiforme |
Cloned (Comment) | Organism |
---|---|
recombinant Escherichia coli expressing sdc converts 40 mM phenol to 10.6 mM salicylate with a 27% (mol/mol) yield at 30°C for 9 h | Cutaneotrichosporon moniliiforme |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM, complete loss of decarboxylation activity, 60% loss of carboxylation activity | Cutaneotrichosporon moniliiforme | |
CuCl2 | 1 mM, 70% loss of carboxylation activity, 14% loss of decarboxylation activity | Cutaneotrichosporon moniliiforme | |
diethyl dicarbonate | 1 mM, 37% inhibition of carboxylation activity, 21% inhibition of decarboxylation activity | Cutaneotrichosporon moniliiforme | |
HgCl2 | 1 mM, complete inhibition of decarboxylation activity, 94% loss of carboxylation activity | Cutaneotrichosporon moniliiforme | |
hydroxylamine | 1 mM, 23% inhibition of carboxylation activity, 9% inhibition of decarboxylation activity | Cutaneotrichosporon moniliiforme | |
additional information | the enzyme is oxygen-insensitive | Cutaneotrichosporon moniliiforme | |
NEM | 1 mM, 28% loss of carboxylation activity, 6% loss of decarboxylation activity | Cutaneotrichosporon moniliiforme | |
NiCl2 | 1 mM, 85% loss carboxylation activity, 46% loss of decarboxylation activity | Cutaneotrichosporon moniliiforme | |
p-chloromercuribenzoic acid | 0.2 mM, 49% loss of carboxylation activity, 82% loss of decarboxylation activity | Cutaneotrichosporon moniliiforme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
salicylate | pH 5.5, 40°C | Cutaneotrichosporon moniliiforme | |
123 | - |
phenol | pH 5.5, 30°C | Cutaneotrichosporon moniliiforme |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1 mM, 1.5fold stimulation of carboxylation activity, no effect on decarboxylation activity | Cutaneotrichosporon moniliiforme |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Cutaneotrichosporon moniliiforme |
140000 | - |
gel filtration | Cutaneotrichosporon moniliiforme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
salicylate | Cutaneotrichosporon moniliiforme | - |
phenol + CO2 | - |
? | |
salicylate | Cutaneotrichosporon moniliiforme WU-0401 | - |
phenol + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutaneotrichosporon moniliiforme | - |
- |
- |
Cutaneotrichosporon moniliiforme WU-0401 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is oxygen insensitive | Cutaneotrichosporon moniliiforme |
Purification (Comment) | Organism |
---|---|
- |
Cutaneotrichosporon moniliiforme |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.47 | - |
pH 5.5, 40°C, production of phenol from salicylic acid | Cutaneotrichosporon moniliiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybenzoate | - |
Cutaneotrichosporon moniliiforme | 1,2-dihydroxybenzene + CO2 | i.e. catechol | r | |
2,3-dihydroxybenzoate | - |
Cutaneotrichosporon moniliiforme WU-0401 | 1,2-dihydroxybenzene + CO2 | i.e. catechol | r | |
2,4-dihydroxybenzoate | i.e beta-resorcylic acid | Cutaneotrichosporon moniliiforme | 1,3-dihydroxybenzene + CO2 | i.e. resorcinol | r | |
2,4-dihydroxybenzoate | i.e beta-resorcylic acid | Cutaneotrichosporon moniliiforme WU-0401 | 1,3-dihydroxybenzene + CO2 | i.e. resorcinol | r | |
2,6-dihydroxybenzoate | i.e gamma-resorcylic acid | Cutaneotrichosporon moniliiforme | 1,3-dihydroxybenzene + CO2 | i.e. resorcinol | r | |
4-aminosalicylate | - |
Cutaneotrichosporon moniliiforme | 3-aminophenol + CO2 | - |
r | |
additional information | the enzyme does not catalyse the carboxylation of cresol. The enzyme does not catalyse the decarboxylation of 3-hydroxybenzoate, 4-hydroxybenzoate, 3,4-dihydroxybenzoate, 5-dihydroxybenzoate, 3-methylsalicylate, 4-methylsalicylate, and vanillic acid (4-hydroxy-3-methoxybenzoate). The substrate recognition of the enzyme for decarboxylation seems to depend strictly on hydroxybenzoic acid with two neighboring hydroxyl and carboxyl groups in the active center of the enzyme | Cutaneotrichosporon moniliiforme | ? | - |
? | |
additional information | the enzyme does not catalyse the carboxylation of cresol. The enzyme does not catalyse the decarboxylation of 3-hydroxybenzoate, 4-hydroxybenzoate, 3,4-dihydroxybenzoate, 5-dihydroxybenzoate, 3-methylsalicylate, 4-methylsalicylate, and vanillic acid (4-hydroxy-3-methoxybenzoate). The substrate recognition of the enzyme for decarboxylation seems to depend strictly on hydroxybenzoic acid with two neighboring hydroxyl and carboxyl groups in the active center of the enzyme | Cutaneotrichosporon moniliiforme WU-0401 | ? | - |
? | |
salicylate | - |
Cutaneotrichosporon moniliiforme | phenol + CO2 | - |
? | |
salicylate | in the reverse direction the enzyme catalyses the regioselective carboxylation of phenol into stoichiometric amounts of salicylic acid | Cutaneotrichosporon moniliiforme | phenol + CO2 | - |
r | |
salicylate | - |
Cutaneotrichosporon moniliiforme WU-0401 | phenol + CO2 | - |
? | |
salicylate | in the reverse direction the enzyme catalyses the regioselective carboxylation of phenol into stoichiometric amounts of salicylic acid | Cutaneotrichosporon moniliiforme WU-0401 | phenol + CO2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 40000, SDS-PAGE | Cutaneotrichosporon moniliiforme |
Synonyms | Comment | Organism |
---|---|---|
salicylic acid decarboxylase | - |
Cutaneotrichosporon moniliiforme |
SCD | - |
Cutaneotrichosporon moniliiforme |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
carboxylation activity | Cutaneotrichosporon moniliiforme |
40 | - |
assay at, decarboxylation activity | Cutaneotrichosporon moniliiforme |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | 20°C: about 35% of maximal activity, 30°C: maximal activity, 40°C: about 10% of maximal activity, production of salicylate from phenol | Cutaneotrichosporon moniliiforme |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
1 h, carboxylation activity is stable up to | Cutaneotrichosporon moniliiforme |
40 | - |
1 h, decarboxylation activity is stable up to, 60% of the carboxylation activity is retained | Cutaneotrichosporon moniliiforme |
50 | - |
1 h, 40% of the decarboxylation activity is retained | Cutaneotrichosporon moniliiforme |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
salicylate | pH 5.5, 40°C | Cutaneotrichosporon moniliiforme | |
1.1 | - |
phenol | pH 5.5, 30°C | Cutaneotrichosporon moniliiforme |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
production of phenol from salicylate | Cutaneotrichosporon moniliiforme |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5.0: about 75% of maximal activity, pH 8.5: about 50% of maximal activity, production of phenol from salicylate | Cutaneotrichosporon moniliiforme |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require pyridoxal 5'-phosphate, NADH, and NADPH | Cutaneotrichosporon moniliiforme |