Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is independent from biotin | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21Star (DE3), BL21 Rosetta, and B834 (DE3) cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
MdcD-MdcE subunits, sitting drop vapor diffusion method, using 0.1 M Bis-Tris (pH 5.8), 18% (w/v) PEG 3350, and 0.2 M ammonium acetate. Holoenzyme in complex with CoA and malonate, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 8% (w/v) tacsimate | Pseudomonas aeruginosa |
the holoenzyme contains four subunits, having an acyl-carrier protein (MdcC subunit) with a distinct prosthetic group, as well as decarboxylase (MdcD-MdcE) and acyl-carrier protein transferase (MdcA) catalytic activities. Structure of the MdcD-MdcE complex at 1.86 A resolution, and overall strcuture of the heterotetramer at 2.2 A resolution | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
Q100E | the mutant of subunit MdcE has a roughly 10fold lower catalytic activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Q100E/Y102F | inactive | Pseudomonas aeruginosa |
S142A | the mutant of subunit MdcE has a roughly 10fold lower catalytic activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Y102F | the mutant of subunit MdcE has about wild type catalytic activity | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas aeruginosa | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate + H+ | Pseudomonas aeruginosa | - |
acetate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I6S7 and Q9I6S6 | beta subunit MdcD and gamma subunit MdcE | - |
Pseudomonas aeruginosa | Q9I6T0 and Q9I6S6 | Q9I6T0 i.e. subunit MdcA, Q9I6S6 i.e. subunit MdcE | - |
Pseudomonas aeruginosa DSM 22644 | Q9I6T0 and Q9I6S6 | Q9I6T0 i.e. subunit MdcA, Q9I6S6 i.e. subunit MdcE | - |
Purification (Comment) | Organism |
---|---|
nickel bead column chromatography and Sephacryl S-300 gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate + H+ | - |
Pseudomonas aeruginosa | acetate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | x-ray crystallography | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
biotin-independent malonate decarboxylase | the holoenzyme contains four subunits having an acyl-carrier protein (MdcC subunit) with a distinct prosthetic group, as well as decarboxylase (MdcD-MdcE) and acyl-carrier protein transferase (MdcA) catalytic activities | Pseudomonas aeruginosa |
MdcACDE | - |
Pseudomonas aeruginosa |