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Literature summary for 4.1.1.72 extracted from

  • Wei, J.; Timler, J.G.; Knutson, C.M.; Barney, B.M.
    Branched-chain 2-keto acid decarboxylases derived from Psychrobacter (2013), FEMS Microbiol. Lett., 346, 105-112.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Lactococcus lactis
expressed in Escherichia coli BL21 cells Psychrobacter cryohalolentis
expressed in Escherichia coli BL21 cells Psychrobacter arcticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, SDS-PAGE Lactococcus lactis
55000
-
x * 55000, SDS-PAGE Psychrobacter cryohalolentis
55000
-
x * 55000, SDS-PAGE Psychrobacter arcticus

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Psychrobacter arcticus
-
-
-
Psychrobacter arcticus 273-4
-
-
-
Psychrobacter cryohalolentis
-
-
-
Psychrobacter cryohalolentis K5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-charged metal chelating column chromatography Lactococcus lactis
nickel-charged metal chelating column chromatography Psychrobacter cryohalolentis
nickel-charged metal chelating column chromatography Psychrobacter arcticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-oxobutyrate
-
Lactococcus lactis 3-methylpropanal + CO2
-
?
3-methyl-2-oxobutyrate
-
Psychrobacter cryohalolentis 3-methylpropanal + CO2
-
?
3-methyl-2-oxobutyrate
-
Psychrobacter arcticus 3-methylpropanal + CO2
-
?
3-methyl-2-oxobutyrate
-
Psychrobacter cryohalolentis K5 3-methylpropanal + CO2
-
?
3-methyl-2-oxobutyrate
-
Psychrobacter arcticus 273-4 3-methylpropanal + CO2
-
?
3-methyl-2-oxopentanoate
-
Lactococcus lactis 2-methylbutanal + CO2
-
?
3-methyl-2-oxopentanoate
-
Psychrobacter cryohalolentis 2-methylbutanal + CO2
-
?
3-methyl-2-oxopentanoate
-
Psychrobacter arcticus 2-methylbutanal + CO2
-
?
3-methyl-2-oxopentanoate
-
Psychrobacter cryohalolentis K5 2-methylbutanal + CO2
-
?
3-methyl-2-oxopentanoate
-
Psychrobacter arcticus 273-4 2-methylbutanal + CO2
-
?
4-methyl-2-oxopentanoate
-
Lactococcus lactis 4-methylbutanal + CO2
-
?
4-methyl-2-oxopentanoate
-
Psychrobacter cryohalolentis 4-methylbutanal + CO2
-
?
4-methyl-2-oxopentanoate
-
Psychrobacter arcticus 4-methylbutanal + CO2
-
?
4-methyl-2-oxopentanoate
-
Psychrobacter cryohalolentis K5 4-methylbutanal + CO2
-
?
4-methyl-2-oxopentanoate
-
Psychrobacter arcticus 273-4 4-methylbutanal + CO2
-
?
Phenylpyruvate
-
Lactococcus lactis Phenylacetaldehyde + CO2
-
?
Phenylpyruvate
-
Psychrobacter cryohalolentis Phenylacetaldehyde + CO2
-
?
Phenylpyruvate
-
Psychrobacter arcticus Phenylacetaldehyde + CO2
-
?
Phenylpyruvate
-
Psychrobacter cryohalolentis K5 Phenylacetaldehyde + CO2
-
?
Phenylpyruvate
-
Psychrobacter arcticus 273-4 Phenylacetaldehyde + CO2
-
?
pyruvate
-
Lactococcus lactis ethanal + CO2
-
?
pyruvate
-
Psychrobacter cryohalolentis ethanal + CO2
-
?
pyruvate
-
Psychrobacter arcticus ethanal + CO2
-
?
pyruvate
-
Psychrobacter cryohalolentis K5 ethanal + CO2
-
?
pyruvate
-
Psychrobacter arcticus 273-4 ethanal + CO2
-
?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Lactococcus lactis
? x * 55000, SDS-PAGE Psychrobacter cryohalolentis
? x * 55000, SDS-PAGE Psychrobacter arcticus

Synonyms

Synonyms Comment Organism
branched-chain 2-keto acid decarboxylase
-
Lactococcus lactis
branched-chain 2-keto acid decarboxylase
-
Psychrobacter cryohalolentis
branched-chain 2-keto acid decarboxylase
-
Psychrobacter arcticus
KDC
-
Psychrobacter cryohalolentis
KDC
-
Psychrobacter arcticus
KdcA
-
Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Psychrobacter arcticus
35
-
-
Psychrobacter cryohalolentis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
phenylpyruvate pH and temperature not specified in the publication Psychrobacter arcticus
1.8
-
phenylpyruvate pH and temperature not specified in the publication Psychrobacter cryohalolentis
7
-
4-methyl-2-oxopentanoate pH and temperature not specified in the publication Psychrobacter arcticus
8.5
-
4-methyl-2-oxopentanoate pH and temperature not specified in the publication Psychrobacter cryohalolentis
23
-
3-methyl-2-oxopentanoate pH and temperature not specified in the publication Psychrobacter arcticus
25
-
3-methyl-2-oxopentanoate pH and temperature not specified in the publication Lactococcus lactis
27
-
3-methyl-2-oxopentanoate pH and temperature not specified in the publication Psychrobacter cryohalolentis
29
-
3-methyl-2-oxobutyrate pH and temperature not specified in the publication Psychrobacter arcticus
33
-
3-methyl-2-oxobutyrate pH and temperature not specified in the publication Psychrobacter cryohalolentis

pH Range

pH Minimum pH Maximum Comment Organism
6 7
-
Lactococcus lactis
6 7
-
Psychrobacter cryohalolentis
6 7
-
Psychrobacter arcticus