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Literature summary for 4.1.1.72 extracted from

  • Berthold, C.L.; Gocke, D.; Wood, M.D.; Leeper, F.J.; Pohl, M.; Schneider, G.
    Structure of the branched-chain keto acid decarboxylase (KdcA) from Lactococcus lactis provides insights into the structural basis for the chemoselective and enantioselective carboligation reaction (2007), Acta Crystallogr. Sect. D, 63, 1217-1224.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is able to catalyze carboligation reactions with an exceptionally broad substrate range, a feature that makes KdcA a potentially valuable biocatalyst for C-C bond formation, in particular for the enzymatic synthesis of diversely substituted 2-hydroxyketones with high enantioselectivity Lactococcus lactis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 25-26% (v/v) PEG 200 in 0.1 M MES buffer pH 6.2 as a precipitant Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
2-[(R)-1-hydroxyethyl]deaza thiamine diphosphate 0.1 mM Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-2-oxobutanoic acid
-
Lactococcus lactis 2-methylpropanal + CO2
-
?
additional information the enzyme is also able to catalyze carboligation reactions with an exceptionally broad substrate range, a feature that makes KdcA a potentially valuable biocatalyst for C-C bond formation, in particular for the enzymatic synthesis of diversely substituted 2-hydroxyketones with high enantioselectivity Lactococcus lactis ?
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Lactococcus lactis

Synonyms

Synonyms Comment Organism
branched-chain keto acid decarboxylase
-
Lactococcus lactis
KdcA
-
Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent Lactococcus lactis