Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is able to catalyze carboligation reactions with an exceptionally broad substrate range, a feature that makes KdcA a potentially valuable biocatalyst for C-C bond formation, in particular for the enzymatic synthesis of diversely substituted 2-hydroxyketones with high enantioselectivity | Lactococcus lactis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 25-26% (v/v) PEG 200 in 0.1 M MES buffer pH 6.2 as a precipitant | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-[(R)-1-hydroxyethyl]deaza thiamine diphosphate | 0.1 mM | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoic acid | - |
Lactococcus lactis | 2-methylpropanal + CO2 | - |
? | |
additional information | the enzyme is also able to catalyze carboligation reactions with an exceptionally broad substrate range, a feature that makes KdcA a potentially valuable biocatalyst for C-C bond formation, in particular for the enzymatic synthesis of diversely substituted 2-hydroxyketones with high enantioselectivity | Lactococcus lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
branched-chain keto acid decarboxylase | - |
Lactococcus lactis |
KdcA | - |
Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent | Lactococcus lactis |